What is the catalog number for Goat anti-RFP Polyclonal antibody?

The catalog number for Goat anti-RFP Polyclonal antibody is AB1140-100.

What sizes are available for Goat anti-RFP Polyclonal antibody?

Goat anti-RFP Polyclonal antibody is available in the following size(s): 300 µg.

Is Goat anti-RFP Polyclonal antibody in stock?

Goat anti-RFP Polyclonal antibody is currently made to order / not held in immediate stock. Contact us for lead time.

Goat anti-RFP Polyclonal antibody

Goat polyclonal antibody to RFP (Red fluorescent protein). RFP is derived from DsRed, an engineered red fluorescent protein from so-called disc corals of the genus Discosoma.

Product Specifications

CAS Number

9007-83-4

Specifications

In 293HEK cells transfected with cds plasmid detects a band of 29 kDa by Western blot. This antibody also recognizes very well tdTomato and does not cross-react to GFP (green fluorescent protein).

Product Name Alternative

Cherry fluorescent protein; dsRed, red fluorescent protein, tdTomato antibody.

UNSPSC Description

Red Fluorescent Protein

Volume

100 µL

Host

Goat

Reactivity

Red Fluorescent Protein (dsRed), mCherry, mOrange, mPlum, mRFP, mStrawberry and tdTomato

Immunogen

Purified recombinant RFP peptide produced in E. coli.

Target Antigen

Purified recombinant RFP peptide produced in E. coli.

Immunogen Type

Recombinant protein

Target

anti-RFP

Clonality

Polyclonal

Isotype

IgG

Conjugation

Unconjugated

Type

Primary

Sequence

MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKGEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAEGRHSTGGMDELYK

Applications

WB, IF, IHC-P, IHC-F, IEM

Purification

Epitope affinity purified

Concentration

3 mg/mL

Dilution

WB:1:500-1:2,000, IF:1:50-1:500, IHC-P:1:50-1:500, IHC-F:1:50-1:500, IEM:1:50-1:500

Form

Polyclonal antibody supplied as a 100 µl (3 mg/mL) aliquot in PBS, 20% glycerol and 0.05% sodium azide. This antibody is epitope-affinity purified from goat antiserum.

Buffer

PBS, 20% glycerol and 0.05% sodium azide

References & Citations

1. D Jiang, D Correa-Gallegos, S Christ, et al. Nature Cell Biology 2018 Apr. PMID:29593327, 2. Tian DS, Peng J, Murugan M, et al. J Neurosci. 2017 Aug. PMID:28716963, 3. Semerci F, Choi WT, Bajic A, et al. Elife. 2017 Jul 12. PMID:28699891

Storage Conditions

For continuous use, store at 2-8 deg;C for one-two days. For extended storage, store in -20 deg;C freezer. Working dilution samples should be discarded if not used within 12 hours.