Goat anti-BAX Polyclonal antibody

Goat polyclonal antibody to BAX. This protein belongs to the BCL2 protein family that act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. The expression of BAX gene is regulated by the tumor suppressor P53 and has been shown to be involved in P53-mediated apoptosis.

Product Specifications

CAS Number

9007-83-4

Specifications

Detects a band of approximately 21 kDa by Western blot in the following cell lysate: hCat, At-T20, MDA-MB-231, H69 and HUH.

Product Name Alternative

BCL2-associated X protein antibody.

UNSPSC Description

BCL2-associated X protein

Volume

200 µL

Gene ID

ENSG00000087088

Accession Number

ENSG00000087088

Host

Goat

Antigen Species

Human

Reactivity

Human, mouse, rat, bovine, canine, chicken/avian, donkey, feline, goat, guinea pig, hamster, horse, porcine, rabbit, sheep, simian, other

Immunogen

Purified recombinant peptide derived from within residues 90 aa to the N-terminus of human BAX produced in E. coli.

Target Antigen

Purified recombinant peptide derived from within residues 90 aa to the N-terminus of human BAX produced in E. coli.

Immunogen Type

Recombinant protein

Target

Anti-BAX

Clonality

Polyclonal

Isotype

IgG

Conjugation

Unconjugated

Type

Primary

Sequence

MDGSGEQPRGGGPTSSEQIMKTGALLLQGFIQDRAGRMGGEAPELALDPVPQDASTKKLSECLKRIGDELDSNMELQRMIAAVDTDSPREVFF

Applications

WB, IHC-P, IHC-F, IF

Purification

Epitope affinity purified

Concentration

2 mg/mL

Dilution

WB:1:500-1:5,000, IF:1:50-1:250, IHC-P:1:250-1:1,000, IHC-F:1:250-1:1,000

Form

Polyclonal antibody supplied as a 200 µl (2 mg/mL) aliquot in PBS, 20% glycerol and 0.05% sodium azide. This antibody is epitope-affinity purified from goat antiserum.

Buffer

PBS, 20% glycerol and 0.05% sodium azide

References & Citations

1. Yu X, He W, Xie J, et al. J Cardiovasc Electrophysiol. 2018 Dec. PMID:30516302

Storage Conditions

For continuous use, store at 2-8 deg;C for one-two days. For extended storage, store in -20 deg;C freezer. Working dilution samples should be discarded if not used within 12 hours.