Goat anti-Ubiquitin Polyclonal antibody

Goat polyclonal antibody to Ubiquitin. Ubiquitin is a highly conserved of about 8.5 kDa regulatory protein expressed in all eukaryotic tissues. Its function is labelling of proteins for degradation through ubiquitin proteasome system. In order to perform this function, the protein to be degraded is first covalently attached to the C terminus of ubiquitin, and a complex of degradative enzymes then recognizes the ubiquitinated complex.

Product Specifications

CAS Number

9007-83-4

Specifications

Using the recombinant human ubiquitin gives a positive signal by Western blot.

Product Name Alternative

HMG20, UBA52, UBA80, UBB, UBC, UBCEP1, UBCEP2, antibody.

UNSPSC Description

Ubiquitin A-52 residue ribosomal protein fusion product 1

Volume

200 µL

Gene ID

ENSG00000221983

Accession Number

ENSG00000221983

Host

Goat

Antigen Species

Human

Reactivity

Human, mouse, rat, bovine, canine, chicken/avian, donkey, feline, goat, guinea pig, hamster, horse, porcine, rabbit, sheep, simian, other

Immunogen

Purified recombinant human ubiquitin produced in E. coli.

Target Antigen

Purified recombinant human ubiquitin produced in E. coli.

Immunogen Type

Recombinant protein

Target

Anti-Ubiquitin

Clonality

Polyclonal

Isotype

IgG

Conjugation

Unconjugated

Type

Primary

Sequence

MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG

Applications

Purification

Epitope affinity purified

Concentration

3 mg/mL

Dilution

WB:1:500-1:2,000

Form

Polyclonal antibody supplied as a 200 µl (3 mg/mL) aliquot in PBS, 20% glycerol and 0.05% sodium azide. This antibody is epitope-affinity purified from goat antiserum.

Buffer

PBS, 20% glycerol and 0.05% sodium azide

Storage Conditions

For continuous use, store at 2-8 deg;C for one-two days. For extended storage, store in -20 deg;C freezer. Working dilution samples should be discarded if not used within 12 hours.