Goat anti-Luciferase Polyclonal antibody
Goat polyclonal antibody to Firefly luciferase. Luciferase is a widely used reporter protein to study gene expression. Luciferase catalyses a bioluminescent reaction which requires luciferin as a substrate, ATP and Mg2+. A cell extract containing luciferase mixed luciferin in the presence of ATP and Mg2+, results in a flash of light that decays rapidly and can be detected by a luminometer. The total light emission is proportional to the luciferase activity of the sample.
Product Specifications
Specifications
In 293HEK cells transfected with cds plasmid detects a band of 62 kDa by Western blot.
Product Name Alternative
Luc and Luciferin 4-monooxygenase antibody.
UNSPSC Description
Firefly Luciferase
Volume
500 µL
Host
Goat
Antigen Species
Firefly
Reactivity
Photinus pyralis
Immunogen
Purified recombinant peptide derived from within residues 340 aa to the C-terminus of Luciferase produced in E. coli.
Target Antigen
Purified recombinant peptide derived from within residues 340 aa to the C-terminus of Luciferase produced in E. coli.
Immunogen Type
Recombinant protein
Target
Anti-Luciferase
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Type
Primary
Sequence
Applications
WB
Purification
Epitope affinity purified
Concentration
3 mg/mL
Dilution
WB:1:500-1:2,000
Form
Buffer
PBS, 20% glycerol and 0.05% sodium azide
Storage Conditions
CAS Number
9007-83-4
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