Goat anti-Luciferase Polyclonal antibody

Goat polyclonal antibody to Firefly luciferase. Luciferase is a widely used reporter protein to study gene expression. Luciferase catalyses a bioluminescent reaction which requires luciferin as a substrate, ATP and Mg2+. A cell extract containing luciferase mixed luciferin in the presence of ATP and Mg2+, results in a flash of light that decays rapidly and can be detected by a luminometer. The total light emission is proportional to the luciferase activity of the sample.

Product Specifications

CAS Number

9007-83-4

Specifications

In 293HEK cells transfected with cds plasmid detects a band of 62 kDa by Western blot.

Product Name Alternative

Luc and Luciferin 4-monooxygenase antibody.

UNSPSC Description

Firefly Luciferase

Volume

200 µL

Host

Goat

Antigen Species

Firefly

Reactivity

Photinus pyralis

Immunogen

Purified recombinant peptide derived from within residues 340 aa to the C-terminus of Luciferase produced in E. coli.

Target Antigen

Purified recombinant peptide derived from within residues 340 aa to the C-terminus of Luciferase produced in E. coli.

Immunogen Type

Recombinant protein

Target

Anti-Luciferase

Clonality

Polyclonal

Isotype

IgG

Conjugation

Unconjugated

Type

Primary

Sequence

LTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMSGYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESILLQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVVFVDEVPKGLTGKLDARKIREILIKAKKGGKSKL

Applications

Purification

Epitope affinity purified

Concentration

3 mg/mL

Dilution

WB:1:500-1:2,000

Form

Polyclonal antibody supplied as a 200 µl (3 mg/mL) aliquot in PBS, 20% glycerol and 0.05% sodium azide. This antibody is epitope-affinity purified from goat antiserum.

Buffer

PBS, 20% glycerol and 0.05% sodium azide

Storage Conditions

For continuous use, store at 2-8 deg;C for one-two days. For extended storage, store in -20 deg;C freezer. Working dilution samples should be discarded if not used within 12 hours.