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Goat anti-Rab27 Polyclonal antibody

Goat polyclonal antibody to mouse Rab27. Rab27 belongs to the small GTPase superfamily, Rab family. The protein is membrane-bound and involved in lysosome-related vesicle transport and small GTPase mediated signal transduction.

Product Specifications

CAS Number

9007-83-4

Specifications

Detects total Rab27 levels by Western blot in the following human, rat and mouse whole cell lysates and transfected cells with GFP-Rab27a and GFP-Rab27b cds.

Product Name Alternative

C25KG, GS2, GTP-binding protein Ram, rab-27, Rab27a, Rab27b, ras-related protein Rab-27A, ras-related protein Rab-27B, RAB27, RAM antibody.

UNSPSC Description

RAB27, member RAS oncogene family

Volume

200 µL

Host

Goat

Antigen Species

Mouse

Reactivity

Human, mouse, rat, bovine, canine, chicken/avian, donkey, feline, goat, guinea pig, hamster, horse, porcine, rabbit, sheep, simian, other

Immunogen

Purified recombinant peptide derived from within residues 120 aa to the C-terminus of mouse Rab27a and rab27b produced in E. coli.

Target Antigen

Purified recombinant peptide derived from within residues 120 aa to the C-terminus of mouse Rab27a and rab27b produced in E. coli.

Immunogen Type

Recombinant protein

Target

Anti-Rab27

Clonality

Polyclonal

Isotype

IgG

Conjugation

Unconjugated

Type

Primary

Applications

WB, IF, IHC-P, IHC-F

Purification

Epitope affinity purified

Concentration

3 mg/mL

Dilution

WB:1:500-1:2,000, IF:1:50-1:250, IHC-P:1:50-1:400, IHC-F:1:50-1:400

Form

​Polyclonal antibody supplied as a 200 or 500 µl (3 mg/mL) aliquot in PBS, 20% glycerol and 0.05% sodium azide. This antibody is epitope-affinity purified from goat antiserum.

Buffer

PBS, 20% glycerol and 0.05% sodium azide

References & Citations

1. Ferreira JV, Soares AR, Ramalho J, et al. Sci Adv 2022 Mar. PMID: 35333565 2. Ferreira JV, Rosa Soares A, Ramalho JS, et al. PLoS One 2019 Oct. PMID:31613922 3. Schorb TS, PhD Thesis, University of Berlin, Germany 2018

Storage Conditions

For continuous use, store at 2-8 deg;C for one-two days. For extended storage, store in -20 deg;C freezer. Working dilution samples should be discarded if not used within 12 hours.

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