Goat anti-Rab11b Polyclonal antibody

Goat polyclonal antibody to mouse Rab11b. Rab11 belongs to the small GTPase superfamily, Rab family. The protein is membrane-bound and plays essential roles in vesicle and granule targeting. Similar to Rab11a, it has been shown that Rab11b associates with recycling endosomes.

Product Specifications

CAS Number

9007-83-4

Specifications

Detects a band of 25 kDa by Western blot in the following human, rat and mouse whole cell lysates and transfected cells with GFP-Rab11b cds. This Ab is specific for Rab11b, it does not recognize Rab11a.

Product Name Alternative

GTP-binding protein YPT3; member of RAS oncogene family; ras-related protein Rab-11B antibody.

UNSPSC Description

RAB11b, member RAS oncogene family

Volume

200 µL

Gene ID

ENSG00000185236

Accession Number

ENSG00000185236

Host

Goat

Antigen Species

Mouse

Reactivity

Human, mouse, rat, bovine, canine, chicken/avian, donkey, feline, goat, guinea pig, hamster, horse, porcine, rabbit, sheep, simian, other

Immunogen

Purified recombinant peptide derived from within residues 120 aa to the C-terminus of mouse Rab11b produced in E. coli.

Target Antigen

Purified recombinant peptide derived from within residues 120 aa to the C-terminus of mouse Rab11b produced in E. coli.

Immunogen Type

Recombinant protein

Target

Anti-Rab11b

Clonality

Polyclonal

Isotype

IgG

Conjugation

Unconjugated

Type

Primary

Sequence

MVGNKSDLRHLRAVPTDEARAFAEKNNLSFIETSALDSTNVEEAFKNILTEIYRIVSQKQIADRAAHDESPGNNVVDISVPPTTDGQRPNKLQCCQSL

Applications

WB, IF, IHC-P, IHC-F

Purification

Epitope affinity purified

Concentration

3 mg/mL

Dilution

WB:1:250-1:2,000, IF:1:50-1:250, IHC-P:1:100-1:400, IHC-F:1:100-1:400

Form

Polyclonal antibody supplied as a 200 µl (3 mg/mL) aliquot in PBS, 20% glycerol and 0.05% sodium azide. This antibody is epitope-affinity purified from goat antiserum.

Buffer

PBS, 20% glycerol and 0.05% sodium azide

References & Citations

1. Cardoso MHS, PhD Thesis, NOVA University of Lisbon, Portugal 2018

Storage Conditions

For continuous use, store at 2-8 deg;C for one-two days. For extended storage, store in -20 deg;C freezer. Working dilution samples should be discarded if not used within 12 hours.