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Goat anti-Rab8 Polyclonal antibody

Goat polyclonal antibody to mouse Rab8. Rab8 belongs to the small GTPase superfamily, Rab family. It has cell-type specific function during the process of exocytosis and coordinates regulation of the cytoskeleton. Rab8 also appears to interface with endocytic recycling circuits. At the molecular level, this GTPase regulates both the export of vesicles from the trans-Golgi apparatus, and the directed translocation along actin filaments and microtubules.

Product Specifications

CAS Number

9007-83-4

Specifications

Detects total Rab8 protein in the following human, rat and mouse whole cell lysates and transfected cells with GFP-Rab8a and GFP-Rab8b cds by Western blot.

Product Name Alternative

RAB8A, RAB8B, RAS-associated protein RAB8A, ras-related protein Rab-8B, ras-related protein Rab-8A, ras-related protein Rab-8B antibody.

UNSPSC Description

RAB8A, member RAS oncogene family

Volume

200 µL

Gene ID

ENSG00000167461

Accession Number

ENSG00000167461

Host

Goat

Antigen Species

Mouse

Reactivity

Human, mouse, rat, bovine, canine, chicken/avian, donkey, feline, goat, guinea pig, hamster, horse, porcine, rabbit, sheep, simian, other

Immunogen

Purified recombinant peptide derived from within residues 107 aa to the C-terminus of mouse Rab8a and Rab8b produced in E. coli.

Target Antigen

Purified recombinant peptide derived from within residues 107 aa to the C-terminus of mouse Rab8a and Rab8b produced in E. coli.

Immunogen Type

Recombinant protein

Target

Anti-Rab8

Clonality

Polyclonal

Isotype

IgG

Conjugation

Unconjugated

Type

Primary

Applications

WB, IF, IHC-P, IHC-F

Purification

Epitope affinity purified

Concentration

3 mg/mL

Dilution

WB:1:250-1:1,000, IF:1:50-1:250, IHC-P:1:250-1:500, IHC-F:1:250-1:500

Form

​Polyclonal antibody supplied as a 200 µl (3 mg/mL) aliquot in PBS, 20% glycerol and 0.05% sodium azide. This antibody is epitope-affinity purified from goat antiserum.

Buffer

PBS, 20% glycerol and 0.05% sodium azide

References & Citations

1. Schiweck J, Murk K, Ledderose J, et al. Nature Communications, 2021. PMID: 33674568, 2. Schiweck J, Murk K, Ledderose J, et al. bioRxiv 2020

Storage Conditions

For continuous use, store at 2-8 deg;C for one-two days. For extended storage, store in -20 deg;C freezer. Working dilution samples should be discarded if not used within 12 hours.

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