Goat anti-Rab5b Polyclonal antibody

Goat polyclonal antibody to mouse Rab5b. Rab5b belongs to the small GTPase superfamily, Rab family. Rab5b is an Early Endosome Marker and functions as a key regulator of vesicular trafficking during early endocytosis.

Product Specifications

CAS Number

9007-83-4

Specifications

Detects Rab5b protein by Western blot in cell lysates of transfected cells with GFP-Rab5b. This Ab is specific for Rab5b; it does not recognize Rab5a and Rab5c.

Product Name Alternative

RAB5B, RAS-associated protein RAB5B, ras-related protein Rab-5B antibody.

UNSPSC Description

RAB5B, member RAS oncogene family

Volume

200 µL

Gene ID

ENSG00000111540

Accession Number

ENSG00000111540

Host

Goat

Antigen Species

Mouse

Reactivity

Human, mouse, rat, bovine, canine, chicken/avian, donkey, feline, goat, guinea pig, hamster, horse, porcine, rabbit, sheep, simian, other

Immunogen

Purified recombinant peptide derived from within residues 115 aa to the C-terminus of mouse Rab5b produced in E. coli.

Target Antigen

Purified recombinant peptide derived from within residues 115 aa to the C-terminus of mouse Rab5b produced in E. coli.

Immunogen Type

Recombinant protein

Target

Anti-Rab5b

Clonality

Polyclonal

Isotype

IgG

Conjugation

Unconjugated

Type

Primary

Sequence

VKELQRQASPSIVIALAGNKADLANKRMVEYEEAQAYADDNSLLFMETSAKTAMNVNDLFLAIAKKLPKSEPQNLGGAAGRSRGVDLHEQSQQNKSQCCSN

Applications

WB, IHC-P, IHC-F, IF

Purification

Epitope affinity purified

Concentration

4 mg/mL

Dilution

WB:1:250-1:1,000, IF:1:50-1:250, IHC-P:1:100-1:500, IHC-F:1:100-1:500

Form

Polyclonal antibody supplied as a 200 µl (4 mg/mL) aliquot in PBS, 20% glycerol and 0.05% sodium azide. This antibody is epitope-affinity purified from goat antiserum.

Buffer

PBS, 20% glycerol and 0.05% sodium azide

References & Citations

1. Cardoso MHS, PhD Thesis, NOVA University of Lisbon, Portugal 2018

Storage Conditions

For continuous use, store at 2-8 deg;C for one-two days. For extended storage, store in -20 deg;C freezer. Working dilution samples should be discarded if not used within 12 hours.