Goat anti-ZO1 ENSG00000104067 Polyclonal antibody

Goat polyclonal to TJP1 tight junction protein 1 (TJP1). TJP1 is located on a cytoplasmic membrane surface of intercellular tight junctions and may be involved in signal transduction at cell-cell junctions.

Product Specifications

CAS Number

9007-83-4

Specifications

Detects endogenous levels of total ZO1 by Western blot in whole cell and tissue lysates.

Product Name Alternative

ZO-1 and zona occludens protein 1 antibody.

UNSPSC Description

Tight junction protein 1

Volume

200 µL

Gene ID

ENSG00000104067

Accession Number

ENSG00000104067

Host

Goat

Antigen Species

Human

Reactivity

Human, mouse, rat, bovine, canine, chicken/avian, donkey, feline, goat, guinea, pig, hamster, horse, porcine, rabbit, sheep, simian, other

Immunogen

Purified recombinant peptide derived from within residues 1580 aa to the C-terminus of human ZO-1 produced in E. coli.

Target Antigen

Purified recombinant peptide derived from within residues 1580 aa to the C-terminus of human ZO-1 produced in E. coli.

Immunogen Type

Recombinant protein

Target

Anti-ZO1

Clonality

Polyclonal

Isotype

IgG

Conjugation

Unconjugated

Type

Primary

Sequence

PQGAIPEGVEQEIYFKVCRDNSILPPLDKEKGETLLSPLVMCGPHGLKFLKPVELRLPHCDPKTWQNKCLPGDPNYLVGANCVSVLIDHF

Applications

WB, IF, IHC-P, IHC-F

Purification

Epitope affinity purified

Concentration

3 mg/mL

Dilution

WB:1:500-1:2,000, IF:1:50-1:250, IHC-P:1:50-1:200, IHC-F:1:50-1:200

Form

Polyclonal antibody supplied as a 200 µl (3 mg/mL) aliquot in PBS, 20% glycerol and 0.05% sodium azide. This antibody is epitope-affinity purified from goat antiserum.

Buffer

PBS, 20% glycerol and 0.05% sodium azide

References & Citations

1. Cardoso MHS, PhD Thesis, NOVA University of Lisbon, Portugal 2018, 2. Pelkonen L, Sato K, Reinisalo M, et al. Mol Pharm. 2017 Mar. Suplemental Material. PMID:28112518

Storage Conditions

For continuous use, store at 2-8 deg;C for one-two days. For extended storage, store in -20 deg;C freezer. Working dilution samples should be discarded if not used within 12 hours.