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Goat anti-TUBA4A Polyclonal antibody

Goat polyclonal antibody to alpha tubulin. Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulin. The alpha and beta tubulins represent the major components of microtubules, while gamma tubulin plays a critical role in the nucleation of microtubule assembly.

Product Specifications

Specifications

This antibody gives a positive signal in the following human (293A, HMEC-1, U-118, HaCat), rat (TR-iBRB), mouse (AtT-20, Hepa), canine (D17) and monkey (COS-7) whole cell lysates.

Product Name Alternative

TUBA1, TUBA4A antibody.

UNSPSC Description

Tubulin, alpha 4a

Volume

500 µL

Gene ID

ENSG00000127824

Accession Number

ENSG00000127824

Host

Goat

Antigen Species

Human

Reactivity

Human, mouse, rat, bovine, canine, chicken/avian, donkey, feline, goat, guinea pig, hamster, horse, porcine, rabbit, sheep, simian, other

Immunogen

Purified recombinant peptide derived from within residues 400 aa to the C-terminus of human alpha tubulin produced in E. coli.

Target Antigen

Purified recombinant peptide derived from within residues 400 aa to the C-terminus of human alpha tubulin produced in E. coli.

Immunogen Type

Recombinant protein

Target

Anti-TUBA4A

Clonality

Polyclonal

Isotype

IgG

Conjugation

Unconjugated

Type

Primary

Sequence

MYAKRAFVHWYVGEGMEEGEFSEAREDMAALEKDYEEVGIDSYEDEDEGEE

Applications

WB, IF

Purification

Epitope affinity purified

Concentration

2 mg/mL

Dilution

WB:1:250-1:2,000, IF:1:50-1:200

Form

Polyclonal antibody supplied as a 200 or 500 µl (2 mg/mL) aliquot in PBS, 20% glycerol and 0.05% sodium azide. This antibody is epitope-affinity purified from goat antiserum.

Buffer

PBS, 20% glycerol and 0.05% sodium azide

References & Citations

1. Ferreira JV, Soares AR, Ramalho J, et al. Sci Adv 2022 Mar. PMID: 35333565 2. Saviano A, De Vita S, Chini MG, et al. Biomolecules 2022 Jan. PMID: 35053247 3. Ferreira JV, Rosa Soares A, Ramalho JS, et al. PLoS One 2019 Oct. PMID:31613922 4. Goncalves SMM, PhD Thesis, University of Minho - Portugal 2015

Storage Conditions

For continuous use, store at 2-8 deg;C for one-two days. For extended storage, store in -20 deg;C freezer. Working dilution samples should be discarded if not used within 12 hours.

CAS Number

9007-83-4

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