Goat anti-P53 Polyclonal antibody

Goat polyclonal antibody to P53. This protein reacts to diverse cellular stresses to regulate target genes that induce apoptosis, senescence, cell cycle arrest, DNA repair, or changes in metabolism. p53 is a DNA-binding protein containing transcription activation, DNA-binding, and oligomerization domains. It is assumed to bind to a p53-binding site and activate expression of downstream genes that inhibit growth and/or invasion, and thus function as a tumour suppressor.

Product Specifications

CAS Number

9007-83-4

Specifications

Detects endogenous levels of P53 by Western blot.

Product Name Alternative

BCC7, LFS1 and TRP53 antibody.

UNSPSC Description

Tumor protein p53

Volume

200 µL

Gene ID

ENSG00000141510

Accession Number

ENSG00000141510

Host

Goat

Antigen Species

Human

Reactivity

Human, mouse, rat, bovine, canine, chicken/avian, donkey, feline, goat, guinea pig, hamster, horse, porcine, rabbit, sheep, simian, other

Immunogen

Purified recombinant peptide derived from within residues 280 aa to the C-terminus of human P53 produced in E. coli.

Target Antigen

Purified recombinant peptide derived from within residues 280 aa to the C-terminus of human P53 produced in E. coli.

Immunogen Type

Recombinant protein

Target

Anti-P53

Clonality

Polyclonal

Isotype

IgG

Conjugation

Unconjugated

Type

Primary

Sequence

DRRTEEENLRKKGEPHHELPPGSTKRALPNNTSSSPQPKKKPLDGEYFTLQIRGRERFEMFRELNEALELKDAQAGKEPGGSRAHSSHLKSKKGQSTSRHKKLMFKTEGPDSD

Applications

WB, IF

Purification

Epitope affinity purified

Concentration

3 mg/mL

Dilution

WB:1:500-1:2,000, IF:1:50-1:250

Form

Polyclonal antibody supplied as a 200 µl (3 mg/mL) aliquot in PBS, 20% glycerol and 0.05% sodium azide. This antibody is epitope-affinity purified from goat antiserum.

Buffer

PBS, 20% glycerol and 0.05% sodium azide

References & Citations

1. Ferreira JV, Rosa Soares A, Ramalho JS, et al. PLoS One. 2019 Oct. PMID:31613922

Storage Conditions

For continuous use, store at 2-8 deg;C for one-two days. For extended storage, store in -20 deg;C freezer. Working dilution samples should be discarded if not used within 12 hours.