Goat anti-HIF1a Polyclonal antibody

Goat polyclonal to alpha subunit of transcription factor hypoxia-inducible factor-1 (HIF-1), which is a heterodimer composed of an alpha and a beta subunit. HIF-1 functions as a master regulator of cellular and systemic homeostatic response to hypoxia by activating transcription of many genes, including those involved in apoptosis, angiogenesis, energy metabolism, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. HIF-1 thus plays an essential role in tumour angiogenesis, embryonic vascularization, and pathophysiology of ischemic disease.

Product Specifications

CAS Number

9007-83-4

Specifications

Detects HIF1a in transfected cells and a band of approximately 120 kDa by Western blot in cell lysates.

Product Name Alternative

alpha subunit (basic helix-loop-helix transcription factor), ARNT interacting protein, basic-helix-loop-helix-PAS protein MOP1, bHLHe78, class E basic helix-loop-helix protein 78, HIF-1alpha, HIF1, HIF1-ALPHA, HIF1a, hypoxia inducible factor 1, hypoxia-inducible factor 1 alpha isoform I.3, hypoxia-inducible factor 1, hypoxia-inducible factor 1-alpha, member of PAS protein 1, member of PAS superfamily 1, MOP1, PASD8, PAS domain-containing protein 8 antibody.

UNSPSC Description

Hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor)

Volume

200 µL

Gene ID

ENSG00000100644

Accession Number

ENSG00000100644

Host

Goat

Antigen Species

Human

Reactivity

Human, mouse, rat, bovine, canine, chicken/avian, donkey, feline, goat, guinea pig, hamster, horse, porcine, rabbit, sheep, simian, other

Immunogen

Purified recombinant peptide derived from within residues 780 aa to the C-terminus of human HIF1a produced in E. coli.

Target Antigen

Purified recombinant peptide derived from within residues 780 aa to the C-terminus of human HIF1a produced in E. coli.

Immunogen Type

Recombinant protein

Target

Anti-HIF1a

Clonality

Polyclonal

Isotype

IgG

Conjugation

Unconjugated

Type

Primary

Sequence

LLGQSMDESGLPQLTSYDCEVNAPIQGSRNLLQGEELLRALDQVN

Applications

Purification

Epitope affinity purified

Concentration

3 mg/mL

Dilution

WB:1:500-1:2,000

Form

Polyclonal antibody supplied as a 200 µl (3 mg/mL) aliquot in PBS, 20% glycerol and 0.05% sodium azide. This antibody is epitope-affinity purified from goat antiserum.

Buffer

PBS, 20% glycerol and 0.05% sodium azide

References & Citations

1. Ferreira JV, Soares AR, Ramalho J, et al. Sci Adv 2022 Mar. PMID: 35333565 2. Martins F, Sacramento JF, Olea E, et al. Antioxidants (Basel) 2021 Aug. PMID: 34439481 3. Rodrigues T, Borges P, Mar L, et al. Pharmacol Res 2020 Sep. PMID:32942016 4. Ferreira JV, Rosa Soares A, Ramalho JS, et al. PLoS One. 2019 Oct. PMID:31613922 5. Rodrigues TDA, PhD Thesis, University of Coimbra, Portugal 2018 6. Fonseca LMO, MSc Thesis, University of Berlin, Germany 2018 7. Rodrigues T, Matafome P, Sereno J, et al. Sci Rep 2017 May 10;7:1698. PMID:28490763 8. Ferreira JV, Soares AR, Ramalho JS, et al. Scientific Reports 5. May 2015. PMID:25958982 9. Sacramento JF, Ribeiro MJ, Rodrigues T, et al. Respir Physiol Neurobiol 2016 Jul. PMID:26993367

Storage Conditions

For continuous use, store at 2-8 deg;C for one-two days. For extended storage, store in -20 deg;C freezer. Working dilution samples should be discarded if not used within 12 hours.