Goat anti-EEA1 Polyclonal antibody

Goat polyclonal antibody to EEA1 – early endosome marker. EEA1 is Early Endosome Antigen 1 protein that binds phospholipid vesicles containing phosphatidylinositol 3-phosphate, which is necessary for endosomal trafficking.

Product Specifications

CAS Number

9007-83-4

Specifications

Detects a band of 180 kDa by Western blot in the following human, mouse and rat whole cell lysates.

Product Name Alternative

Early endosome antigen 1, 162kD; early endosome-associated protein; endosome-associated protein p162; zinc finger FYVE domain-containing protein 2 antibody.

UNSPSC Description

Early Endosome Antigen 1

Volume

200 µL

Gene ID

ENSG00000102189

Accession Number

ENSG00000102189

Host

Goat

Antigen Species

Human

Reactivity

Human, mouse, rat, bovine, canine, chicken/avian, donkey, feline, goat, guinea pig, hamster, horse, porcine, rabbit, sheep, simian, other

Immunogen

Purified recombinant peptide within residues 1230 aa to the C-terminus of human EEA1 produced in E. coli.

Target Antigen

Purified recombinant peptide within residues 1230 aa to the C-terminus of human EEA1 produced in E. coli.

Immunogen Type

Recombinant protein

Target

Anti-EEA1

Clonality

Polyclonal

Isotype

IgG

Conjugation

Unconjugated

Type

Primary

Sequence

KKHEENEAKLTMQITALNENLGTVKKEWQSSQRRVSELEKQTDDLRGEIAVLEATVQNNQDERRALLERCLKGEGEIEKLQTKVLELQRKLDNTTAAVQELGRENQSLQIKHTQALNRKWAEDNEVQNCMACGKGFSVTVRRHHCRQCGNIFCAECSAKNALTPSSKKPVRVCDACFNDLQG

Applications

WB, IF, IHC-F

Purification

Epitope affinity purified

Concentration

3 mg/mL

Dilution

WB:1:250-1:1,000, IF:1:25-1:250, IHC-F:1:100-1:500

Form

Polyclonal antibody supplied as a 200 µl (3 mg/mL) aliquot in PBS, 20% glycerol and 0.05% sodium azide. This antibody is epitope-affinity purified from goat antiserum.

Buffer

PBS, 20% glycerol and 0.05% sodium azide

References & Citations

1. Ferreira JV, Soares AR, Ramalho J, et al. Sci Adv 2022 Mar. PMID: 35333565 2. Ernst K, Landenberger M, Nieland J, et al. Toxins (Basel) 2021 May. PMID: 34071730

Storage Conditions

For continuous use, store at 2-8 deg;C for one-two days. For extended storage, store in -20 deg;C freezer. Working dilution samples should be discarded if not used within 12 hours.