Goat anti-CX43 Polyclonal antibody

Goat polyclonal to connexin 43, which is a member of the connexin family. Cx43 is a component of gap junctions, which are composed of arrays of intercellular channels that provide a route for the diffusion of low molecular weight materials from cell to cell. This protein is the major protein of gap junctions in embryonic development and in the heart that are thought to have a crucial role in the synchronized contraction of the heart.

Product Specifications

CAS Number

9007-83-4

Specifications

Detects endogenous levels of total connexin 43.

Product Name Alternative

AVSD3, CX43, DFNB38, gap junction protein, alpha 1, 43kDa, GJA1, GJAL HSS, HLHS1, ODDD antibody.

UNSPSC Description

Gap junction protein, alpha 1, 43kDa

Volume

200 µL

Gene ID

ENSG00000152661

Accession Number

ENSG00000152661

Host

Goat

Antigen Species

Human

Reactivity

Human, mouse, rat, bovine, canine, chicken/avian, donkey, feline, goat, guinea pig, hamster, horse, porcine, rabbit, sheep, simian, other

Immunogen

Purified recombinant peptide derived from within residues 230 aa to the C-terminus of rat CX43 produced in E. coli.

Target Antigen

Purified recombinant peptide derived from within residues 230 aa to the C-terminus of rat CX43 produced in E. coli.

Immunogen Type

Recombinant protein

Target

Anti-CX43

Clonality

Polyclonal

Isotype

IgG

Conjugation

Unconjugated

Type

Primary

Sequence

VKDRVKGRSDPYHATTGPLSPSKDCGSPKYAYFNGCSSPTAPLSPMSPPGYKLVTGDRNNSSCRNYNKQASEQNWANYSAEQNRMGQAGSTISNSHAQPFDFPDDNQNAKKVAAGHELQPLAIVDQRPSSRASSRASSRPRPDDLEI

Applications

WB, IF, IHC-P, IHC-F

Purification

Epitope affinity purified

Concentration

4 mg/mL

Dilution

WB:1:500-1:2,000, IF:1:50-1:250, IHC-P:1:250-1:800, IHC-F:1:250-1:800

Form

Polyclonal antibody supplied as a 200 or 500 µl (4 mg/mL) aliquot in PBS, 20% glycerol and 0.05% sodium azide. This antibody is epitope-affinity purified from goat antiserum and it is identical to AB1600.

Buffer

PBS, 20% glycerol and 0.05% sodium azide

References & Citations

1. Martins-Marques T, Costa MC, Catarino S, et al. EMBO Rep 2022 May. PMID: 35593040 2. Lissoni A, Hulpiau P, Martins-Marques T, et al. Cardiovasc Res 2021 Jan. PMID: 31841141 3. Catarino S, Ribeiro-Rodrigues TM, Sa Ferreira R, et al. Cells 2020 Apr. PMID: 32272685 4. Wang W, Ye S, Zhang L, et al. BMC Cardiovasc Disord 2020. PMID:32066388 5. Barbeitos JP, MSc Thesis, University of Coimbra, Portugal 2018 6. Martins-Marques T, Pinho MJ, Zuzarte M. et al. J Extracell Vesicles 2016 Sep. PMID:27702427 7. Paiva RA, MSc Thesis, University of Coimbra, Portugal 2015 8. Ferreira RRS, MSc Thesis, University of Coimbra, Portugal 2015 9. Martins-Marques T, Anjo SI, Pereira P, et al. Mol Cell Proteomics 2015 Aug. PMID:26316108 Supplemental Information 10. Ribeiro-Rodrigues TM, Catarino S, Marques C, et al. FASEB J 2014 Nov. PMID:25070368

Storage Conditions

For continuous use, store at 2-8 deg;C for one-two days. For extended storage, store in -20 deg;C freezer. Working dilution samples should be discarded if not used within 12 hours.