Goat anti-CD63 Polyclonal antibody

CD63 is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of the members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. These proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. This protein is a cell surface glycoprotein that is known to complex with integrins. It may function as a blood platelet activation marker.

Product Specifications

CAS Number

9007-83-4

Specifications

Reacts with CD63, a 40-60 kDa glycoprotein, detected by Western blot in the following human (HeLa, HUH, Jurkat), mouse (AtT-20, Hepa, 3T3, RAW264.7), canine (MDCK) and monkey (COS-7) whole cell lysates.

Product Name Alternative

CD63 antigen, CD63 antigen (melanoma 1 antigen), granulophysin, LAMP-3, lysosomal-associated membrane protein 3, lysosome-associated membrane glycoprotein 3, ME491, melanoma-associated antigen, melanoma 1 antigen, melanoma-associated antigen ME491, mLA1, ocular melanoma-associated antigen, OMA81H, tetraspanin-30, TSPAN30 antibody.

UNSPSC Description

Lysosome-associated membrane glycoprotein 3

Volume

500 µL

Gene ID

ENSG00000135404

Accession Number

ENSG00000135404

Host

Goat

Antigen Species

Human

Reactivity

Human, mouse, rat, bovine, canine, chicken/avian, donkey, feline, goat, guinea pig, hamster, horse, porcine, rabbit, sheep, simian, other

Immunogen

Purified recombinant peptide derived from within residues 120 aa to 175 aa of human CD63 produced in E. coli.

Target Antigen

Purified recombinant peptide derived from within residues 120 aa to 175 aa of human CD63 produced in E. coli.

Immunogen Type

Recombinant protein

Target

Anti-CD63

Clonality

Polyclonal

Isotype

IgG

Conjugation

Unconjugated

Type

Primary

Sequence

MENYPKNNHTASILDRMQADFKCCGAANYTDWEKIPSMSKNRVPDSCCI

Applications

WB, IF, IHC-P, IHC-F

Purification

Epitope affinity purified

Concentration

3 mg/mL

Dilution

WB:1:500-1:5,000, IF:1:25-1:250, IHC-P:1:250-1:1,000, IHC-F:1:250-1:1,000

Form

Polyclonal antibody supplied as a 200 or 500 µl (3 mg/mL) aliquot in PBS, 20% glycerol and 0.05% sodium azide. This antibody is epitope-affinity purified from goat antiserum.

Buffer

PBS, 20% glycerol and 0.05% sodium azide

References & Citations

1. Amorim M, Martins B, Caramelo F, et al. Front Med (Lausanne) 2022 May. PMID: 35692536 2. Matsui T, Sakamaki Y, Nakashima S, et al. Cell Rep 2022 May. PMID: 35649370 3. Martins-Marques T, Costa MC, Catarino S, et al. EMBO Rep 2022 May. PMID: 35593040 4. Ferreira JV, Soares AR, Ramalho J, et al. Sci Adv 2022 Mar. PMID: 35333565 5. Saludas L, Garbayo E, Ruiz-Villalba A, et al. Eur J Pharm Biopharm 2021 Dec. PMID: 34968647 6. Garbayo E, Ruiz-Villalba A, Hernandez SC, et al. Acta Biomater 2021 May. PMID: 33716175 7. Aires ID, Ribeiro-Rodrigues T, Boia R, et al. Glia 2020 Dec. PMID: 32645245 8. Martins-Marques T, Ribeiro-Rodrigues T, de Jager SC, et al. Life Sci Alliance 2020 Oct. PMID: 33097557 9. Armando F, Gambini M, Corradi A, et al. Viruses 2020 Feb. PMID: 32054075 10. Simpong LD, PhD Thesis Universität Leipzig, Germany 2020 11. Carvalho AS, Moraes MCS, Hyun Na C, et al. Cancers 2020 Nov. PMID: 33233545 12. Ferreira JV, Rosa Soares A, Ramalho JS, et al. PLoS One 2019 Oct. PMID:31613922 13. Cardoso MHS, PhD Thesis, NOVA University of Lisbon, Portugal 2018 14. Martins-Marques T, Pinho MJ, Zuzarte M. et al. J Extracell Vesicles 2016 Sep 29;5:32538. PMID:27702427 15. Successfully validated (Western Blotting) by Center for Diabetes and Metabolic Diseases, Indiana University, US

Storage Conditions

For continuous use, store at 2-8 deg;C for one-two days. For extended storage, store in -20 deg;C freezer. Working dilution samples should be discarded if not used within 12 hours.