Goat anti-Clathrin HC Polyclonal antibody

Goat polyclonal to Clathrin Heavy Chain. Clathrin is formed by three clathrin heavy chains and three light chains. This protein is a major protein component of the cytoplasmic face of intracellular organelles, called coated vesicles and coated pits. These specialized organelles are involved in endocytosis of a variety of macromolecules and in the intracellular trafficking of receptors.

Product Specifications

CAS Number

9007-83-4

Specifications

Detects endogenous levels of total CLTC by Western blot in the whole cell lysates (MDCK, At-T20, COS-7).

Product Name Alternative

clathrin, clathrin heavy chain 1, clathrin heavy polypeptide (Hc), clathrin heavy polypeptide-like 2, CLTC, CHC17, CLH-17, CLTCL2, Hc, heavy chain (Hc) antibody.

UNSPSC Description

Clathrin, heavy chain (Hc)

Volume

200 µL

Gene ID

ENSG00000141367

Accession Number

ENSG00000141367

Host

Goat

Antigen Species

Human

Reactivity

Human, mouse, rat, bovine, canine, chicken/avian, donkey, feline, goat, guinea pig, hamster, horse, porcine, rabbit, sheep, simian, other

Immunogen

Purified recombinant peptide derived from within residues 50 aa to N-terminus of human CLTC produced in E. coli.

Target Antigen

Purified recombinant peptide derived from within residues 50 aa to N-terminus of human CLTC produced in E. coli.

Immunogen Type

Recombinant protein

Target

Anti-Clathrin HC

Clonality

Polyclonal

Isotype

IgG

Conjugation

Unconjugated

Type

Primary

Sequence

MAQILPIRFQEHLQLQNLGINPANIGFSTLTMESDKFICIREKVGEQAQVVII

Applications

WB, IF

Purification

Epitope affinity purified

Concentration

2 mg/mL

Dilution

WB:1:250-1:1,000, IF:1:25-1:250

Form

Polyclonal antibody supplied as a 200 µl (2 mg/mL) aliquot in PBS, 20% glycerol and 0.05% sodium azide. This antibody is epitope-affinity purified from goat antiserum.

Buffer

PBS, 20% glycerol and 0.05% sodium azide

References & Citations

1. Imbastari F, PhD Thesis, Humboldt-Universität zu Berlin, Germany 2020 2. Caì Y, Postnikova EN, Bernbaum JG, et al. J Virol. 2015 Jan. PMID: 25355889

Storage Conditions

For continuous use, store at 2-8 deg;C for one-two days. For extended storage, store in -20 deg;C freezer. Working dilution samples should be discarded if not used within 12 hours.