Goat anti-Cathepsin D Polyclonal antibody

Goat polyclonal antibody to Cathepsin D. Cathepsin D is a lysosomal aspartic protease of the pepsin family. Acid protease active in intracellular protein breakdown. It is composed of a dimer of disulphide-linked heavy and light chains, both produced from a single protein precursor. It is involved in the pathogenesis of several diseases such as breast cancer and possibly Alzheimer disease.

Product Specifications

CAS Number

9007-83-4

Specifications

This antibody gives a positive signal in the following human (Jurkat, HT1080, HUH, MDA-MB-231, ARPE19, SH-SY5Y), canine (MDCK) and monkey (COS-7) whole cell lysates.

Product Name Alternative

ceroid-lipofuscinosis, CLN10; CPSD, CTSD, lysosomal aspartyl protease, lysosomal aspartyl peptidase, neuronal 10 antibody.

UNSPSC Description

Cathepsin D

Volume

200 µL

Gene ID

ENSG00000117984

Accession Number

ENSG00000117984

Host

Goat

Antigen Species

Human

Reactivity

Human, mouse, rat, bovine, canine, chicken/avian, donkey, feline, goat, guinea pig, hamster, horse, porcine, rabbit, sheep, simian, other

Immunogen

Purified recombinant peptide derived from within residues 275 aa to the C-terminus of human Cathepsin D produced in E. coli.

Target Antigen

Purified recombinant peptide derived from within residues 275 aa to the C-terminus of human Cathepsin D produced in E. coli.

Immunogen Type

Recombinant protein

Target

Anti-Cathepsin D

Clonality

Polyclonal

Isotype

IgG

Conjugation

Unconjugated

Type

Primary

Sequence

DQVEVASGLTLCKEGCEAIVDTGTSLMVGPVDEVRELQKAIGAVPLIQGEYMIPCEKVSTLPAITLKLGGKGYKLSPEDYTLKVSQAGKTLCLSGFMGMDIPPPSGPLWILGDVFIGRYYTVFDRDNNRVGFAEAARL

Applications

WB, IF, IHC-P, IHC-F, IEM

Purification

Epitope affinity purified

Concentration

3 mg/mL

Dilution

WB:1:250-1:1,000, IF:1:50-1:200, IHC-P:1:200-1:1,000, IHC-F:1:200-1:1,000, IEM:1:50-1:200

Form

Polyclonal antibody supplied as a 200 or 500 µl (3 mg/mL) aliquot in PBS, 20% glycerol and 0.05% sodium azide. This antibody is epitope-affinity purified from goat antiserum.

Buffer

PBS, 20% glycerol and 0.05% sodium azide

References & Citations

1. Romagnoli M, Bresson L, Di-Cicco A, et al. Development 2020 Jan. PMID:31988184, 2. Pelkonen L, Sato K, Reinisalo M, et al. Mol Pharm 2017 Mar 6. PMID:28112518

Storage Conditions

For continuous use, store at 2-8 deg;C for one-two days. For extended storage, store in -20 deg;C freezer. Working dilution samples should be discarded if not used within 12 hours.