Goat anti-CANX Polyclonal antibody

Goat polyclonal to CANX (Calnexin) - endoplasmic reticulum (ER) membrane marker. CANX is a member of the Calnexin family of molecular chaperones. This protein is a calcium-binding, ER-associated protein that interacts transiently with newly synthesized N-linked glycoproteins, facilitating protein folding and assembly. It may also play a central role in the quality control of protein folding by retaining incorrectly folded protein subunits within the ER for degradation.

Product Specifications

CAS Number

9007-83-4

Specifications

Detects a band of 90 kDa by Western blot in the following human (293A, primary fibroblasts, HaCat, HeLa, HMEC-1, Jurkat, MNT1, U-118, rat (TR-iBRB), mouse (3T3, AtT-20, Hepa, Raw264.7), monkey (COS-7) and canine (D17) whole cell lysates.

Product Name Alternative

Calnexin, CALX, CNX, FLJ26570, histocompatibility complex class I antigen binding protein p88, IP90, major histocompatibility complex class I antigen-binding protein p88, MS952, P90 antibody.

UNSPSC Description

Calnexin

Volume

200 µL

Gene ID

Gene ID: 821, ENSG00000127022

Accession Number

Gene ID: 821, ENSG00000127022

Host

Goat

Antigen Species

Human

Reactivity

Human, mouse, rat, bovine, canine, chicken/avian, donkey, feline, goat, guinea pig, hamster, horse, porcine, rabbit, sheep, simian, other

Immunogen

Purified recombinant peptide within residues 550 aa to the C-terminus of human CANX produced in E. coli.

Target Antigen

Purified recombinant peptide within residues 550 aa to the C-terminus of human CANX produced in E. coli.

Immunogen Type

Recombinant protein

Target

Anti-CANX

Clonality

Polyclonal

Isotype

IgG

Conjugation

Unconjugated

Type

Primary

Sequence

KSDAEEDGGTVSQEEEDRKPKAEEDEILNRSPRNRKPRRE

Applications

WB, IF, IHC-P, IHC-F

Purification

Epitope affinity purified

Concentration

2 mg/mL

Dilution

WB:1:500-1:5,000, IF:1:50-1:500, IHC-P:1:200-1:1,000, IHC-F:1:200-1:1,000

Form

Polyclonal antibody supplied as a 200 or 500 µl (2 mg/mL) aliquot in PBS, 20% glycerol and 0.05% sodium azide. This antibody is epitope-affinity purified from goat antiserum.

Buffer

PBS, 20% glycerol and 0.05% sodium azide

References & Citations

1. Amaro A, Sousa D, Sá-Rocha M, et al. Life Sci 2023 Mar. PMID: 36948389 2. Gil-Cabrerizo P, Saludas L, Felipe Prósper F et al. Int J Pharm 2022 Dec. PMID: 36332831 3. Tavares G, Rosendo-Silva D, Simões F, et al. Int J Mol Sci 2023 Jan. PMID: 36768789 4. Saludas L, Garbayo E, Ruiz-Villalba A, et al. Eur J Pharm Biopharm 2022 Jan. PMID: 34968647 5. Schulte A, Bieniussa L, Gupta R, et al. Cell Calcium 2022 Jan. PMID: 34896701 6. Alves LS, Marques ARA, Padrao N, et al. J Cell Sci 2021 Sep. PMID: 34528688 7. Saviano A, De Vita S, Chini MG, et al. Biomolecules 2021 Oct. PMID: 34680124 8. Saludas L, Garbayo E, Ruiz-Villalba A, et al. Eur J Pharm Biopharm 2021 Dec. PMID: 34968647 1. Ghanawi H, Hennlein L, Zare A, et al. Nucleic Acids Res 2021 Dec. PMID: 34850154 2. Monteiro-Alfredo T, Caramelo B, Arbeláez D, et al. Nutrients 2021 Aug. PMID: 34578832 3. Monteiro-Alfredo T, Oliveira S, Amaro A, et al. Nutrients 2021 Aug. PMID: 34445015 4. Selezneva A, Yoshida M, Gibb A, et al. Pharmacological Reports 2021. PMID: 33713314 5. Martinho JM, Coelho A, Oliveiros B, et al. Int Endod J 2021 May. PMID:33999433 6. Tavares G, Marques D, Barra C, et al. Mol Metab 2021 Feb. PMID: 33933677 7. Garbayo E, Ruiz-Villalba A, Hernandez SC, et al. Acta Biomaterialia 2021 8. Rodrigues T, Borges P, Mar L, et al. Pharmacol Res 2020 Sep. PMID: 32942016 9. Sousa FJ, Correia RG, Cruz AF, et al. Brain, Behavior, & Immunity - Health 2020 May 10. Monteiro-Alfredo T, Matafome P, Iacia BP, et al. Oxid Med Cell Longev 2020 Mar. PMID: 3225695 11. Tejero R, Balk S, Franco-Espin J, et al. iScience. 2020 Feb. PMID: 31981925 12. Graça SSF. MSc Thesis, Universidade do Algarve, Portugal 2019 13. Balk SM, PhD Thesis University of Wurzburg, Germany 2019 14. Fricke S, Metzdorf K, Ohm M, et al. Cell Rep 2019 Oct. PMID:31618635 15. Neves C, Rodrigues T, Sereno J, et al. Oxid Med Cell Longev 2019 Jun. PMID:31341532 16. Fonseca LMO, MSc Thesis, University of Coimbra, Portugal 2018 17. Silva MM, Gomes-Alves P, Rosa S, et al. J Biotechnol 2018 Aug. PMID:30165116 18. Rodrigues TDA, PhD Thesis, University of Coimbra, Portugal 2018 19. Ribeiro M, Castelhano J, Petrella LI, et al. J Magn Reson Imaging 2018 Jan. PMID:29377412 20. Awadh A, PhD Thesis, University of Alberta, Canada 2018 21. Ribeiro STF, PhD Thesis University of Lisbon, Portugal 2017 22. Rodrigues T, Matafome P, Sereno J, et al. Sci Rep 2017 May. PMID:28490763 23. Cabral AMD, MSc Thesis, University of Lisbon 2017 24. Thieleke-Matos C, Lopes da Silva M, Cabrita-Santos L et al. Cellular Microbiology 2016 Mar. PMID:26399761 25. Sivadasan R, PhD Thesis, Wurzburg University 2016 26. Neves CAF, MSc Thesis University of Aveiro, Portugal 2015

Storage Conditions

For continuous use, store at 2-8 deg;C for one-two days. For extended storage, store in -20 deg;C freezer. Working dilution samples should be discarded if not used within 12 hours.