Goat anti-CANX Polyclonal antibody

Goat polyclonal to CANX (Calnexin) - endoplasmic reticulum (ER) membrane marker. CANX is a member of the calnexin family of molecular chaperones. This protein is a calcium-binding, ER-associated protein that interacts transiently with newly synthesized N-linked glycoproteins, facilitating protein folding and assembly. It may also play a central role in the quality control of protein folding by retaining incorrectly folded protein subunits within the ER for degradation.

Product Specifications

CAS Number

9007-83-4

Specifications

Detects a band of 90 kDa by Western blot in the following human (293A, primary fibroblasts, HaCat, HeLa, HMEC-1, Jurkat, MNT1, U-118, rat (TR-iBRB), mouse (3T3, AtT-20, Hepa, Raw264.7), monkey (COS-7) and canine (D17) whole cell lysates.

Product Name Alternative

Calnexin, CALX, CNX, FLJ26570, histocompatibility complex class I antigen binding protein p88, IP90, major histocompatibility complex class I antigen-binding protein p88, MS952, P90 antibody.

UNSPSC Description

Calnexin

Volume

200 µL

Gene ID

Gene ID: 821, ENSG00000127022

Accession Number

Gene ID: 821, ENSG00000127022

Host

Goat

Antigen Species

Human

Reactivity

Human, mouse, rat, bovine, canine, chicken/avian, donkey, feline, goat, guinea pig, hamster, horse, porcine, rabbit, sheep, simian, other

Immunogen

Purified recombinant peptide within residues 550 aa to the C-terminus of human CANX produced in E. coli.

Target Antigen

Purified recombinant peptide within residues 550 aa to the C-terminus of human CANX produced in E. coli.

Immunogen Type

Recombinant protein

Target

Anti-CANX

Clonality

Polyclonal

Isotype

IgG

Conjugation

Unconjugated

Type

Primary

Sequence

KSDAEEDGGTVSQEEEDRKPKAEEDEILNRSPRNRKPRRE

Applications

WB, IF, IHC-P, IHC-F

Purification

Epitope affinity purified

Concentration

2 mg/mL

Dilution

WB:1:500-1:5,000, IF:1:50-1:500, IHC-P:1:200-1:1,000, IHC-F:1:200-1:1,000

Form

Polyclonal antibody supplied as a 200 or 500 µl (2 mg/mL) aliquot in PBS, 20% glycerol and 0.05% sodium azide. This antibody is epitope-affinity purified from goat antiserum and it is identical to AB4100-200.

Buffer

PBS, 20% glycerol and 0.05% sodium azide

References & Citations

1. Martins-Marques T, Costa MC, Catarino S, et al. EMBO Rep 2022 May. PMID: 35593040 2. Ferreira JV, Soares AR, Ramalho J, et al. Sci Adv 2022 Mar. PMID: 35333565 3. Moniz I, Ramalho-Santos J, Branco AF. Int J Mol Sci 2022 Mar. PMID: 35409106 4. Martins FO, Sacramento JF, Olea E, et al. Antioxidants (Basel) 2021 Aug. PMID: 34439481 5. Melo BF, Prieto-Lloret J, Cabral MD, et al. Sci Rep 2021 Mar. PMID: 33723367 6. Lissoni A, Hulpiau P, Martins-Marques T, et al. Cardiovasc Res 2021 Jan. PMID: 31841141 7. Aires ID, Ribeiro-Rodrigues T, Boia R, et al. Glia 2020 Dec. PMID: 32645245 8. Martins-Marques T, Ribeiro-Rodrigues T, de Jager SC, et al. Life Sci Alliance 2020 Oct. PMID: 33097557 9. Ferreira-Silva J, Aires ID, Boia R, et al. Int J Mol Sci 2020 Sep.MID: 33007835 10. Sousa FJ, Correia RG, Cruz AF, et al. Brain, Behavior, & Immunity 2020 Apr. PMID: 34589855 11. Encarnacao M, Coutinho MF, Cho SM, et al. Mol Genet Genomic Med 2020 Sep. PMID: 32931663 12. Catarino S, Ribeiro-Rodrigues TM, Sa Ferreira R, et al. Cells 2020 Apr. PMID: 32272685 13. Sacramento JF, Martins FO, Rodrigues T, et al. Front Endocrinol (Lausanne) 2020 Apr. PMID:32411098 14. Boia R, Dias PAN, Martins JM, et al. J Control Release 2019 Nov. PMID:31715277 15. Ferreira JV, Rosa Soares A, Ramalho JS, et al. PLoS One 2019 Oct. PMID:31613922 16. Sacramento JF, Olea E, Ribeiro MJ, et al. J Physiol 2019 Aug. PMID:31426127 17. Melo BF, Sacramento JF, Ribeiro MJ, et al. Nutrients 2019 May.PMID:31141900 18. Sanzà P, Evans RD, Briggs DA, et al. J Cell Sci 2019 Apr. PMID:30898842 19. Barbeitos JP, MSc Thesis, University of Coimbra, Portugal 2018 20. Cardoso MHS, PhD Thesis, NOVA University of Lisbon, Portugal 2018 21. Ribeiro ST, Tesio M, Ribot JC, et al. Leukemia 2017 Jul. PMID:27899804 22. Pelkonen L, Sato K, Reinisalo M, et al. Mol Pharm 2017 Mar 6. PMID:28112518 23. Martins-Marques T, Pinho MJ, Zuzarte M. et al. J Extracell Vesicles 2016 Sep. PMID:27702427 24. Neves MMM, MSc Thesis, University of Coimbra, Portugal 2015 25. Ferreira RRS, MSc Thesis, Coimbra University 2015 26. Paiva RA, MSc Thesis, Coimbra University 2015 27. Samtleben S, PhD Thesis, Universität Würzburg 2014 28. Ribot JC, Ribeiro ST, Correia DV, Sousa AE, Silva-Santos B. J Immuno 2014 Mar, PMID: 24489097 29. Marques, CIR, MSc Thesis, Coimbra University 2013

Storage Conditions

For continuous use, store at 2-8 deg;C for one-two days. For extended storage, store in -20 deg;C freezer. Working dilution samples should be discarded if not used within 12 hours.