Goat anti-ATG12 Polyclonal antibody

Goat polyclonal antibody to ATG12 - autophagosome marker. Autophagy is a process of bulk protein degradation in which cytoplasmic components, including organelles, are enclosed in double-membrane structures called autophagosomes and delivered to lysosomes or vacuoles for degradation. ATG12 is the human homolog of a yeast protein involved in autophagy.

Product Specifications

CAS Number

9007-83-4

Specifications

Detects GFP-ATG12 in transfected cells by Western blot.

Product Name Alternative

APG12, Apg12 (autophagy, yeast) homolog, APG12L, FBR93, HAPG12, autophagy protein 12, autophagy-related protein 12 antibody.

UNSPSC Description

Autophagy related 12

Volume

200 µL

Gene ID

ENSG00000145782

Accession Number

ENSG00000145782

Host

Goat

Antigen Species

Human

Reactivity

Human, mouse, rat, bovine, canine, chicken/avian, donkey, feline, goat, guinea, pig, hamster, horse, porcine, rabbit, sheep, simian, other

Immunogen

Purified recombinant peptide within residues 65 aa to the N-terminus of human ATG12 produced in E. coli.

Target Antigen

Purified recombinant peptide within residues 65 aa to the N-terminus of human ATG12 produced in E. coli.

Immunogen Type

Recombinant protein

Target

Anti-ATG12

Clonality

Polyclonal

Isotype

IgG

Conjugation

Unconjugated

Type

Primary

Sequence

AEEPQSVLQLPTSIAAGGEGLTDVSPETTTPEPPSSAAVSPGTEEPAGD

Applications

WB, IF

Purification

Epitope affinity purified

Concentration

3 mg/mL

Dilution

WB:1:500-1:2,000, IF:1:50-1:250, IHC-P:1:200-1:1,000, IHC-F:1:200-1:1,000

Form

Polyclonal antibody supplied as a 200 µl (3 mg/mL) aliquot in PBS, 20% glycerol and 0.05% sodium azide. This antibody is epitope-affinity purified from goat antiserum.

Buffer

PBS, 20% glycerol and 0.05% sodium azide

References & Citations

1. Kharaziha P, Panaretakis T. Methods Enzymol. 2017. PMID:28253959

Storage Conditions

For continuous use, store at 2-8 deg;C for one-two days. For extended storage, store in -20 deg;C freezer. Working dilution samples should be discarded if not used within 12 hours.