Goat anti-GST Polyclonal antibody

Goat polyclonal antibody to GST (Glutathione-S-Transferase). GST is a 26 kDa protein encoded by the parasitic helminth Schistosoma japonicum and widely used in GST plasmid expression vectors as a fusion protein with foreign protein.

Product Specifications

CAS Number

9007-83-4

Specifications

In lysates of E. coli cells transformed with pGEX plasmid detects a band of 26 kDa by Western blot. The specificity of this Ab is identical to AB99919.

UNSPSC Description

Gluthatione-S-Transferase

Volume

200 µL

Host

Goat

Reactivity

GST

Immunogen

Purified recombinant protein produced in E. coli.

Target Antigen

Purified recombinant protein produced in E. coli.

Immunogen Type

Recombinant protein

Target

anti-GST

Clonality

Polyclonal

Isotype

IgG

Conjugation

Unconjugated

Type

Primary

Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNmLGGCPKERAEISmLEGAVLDIRYGVSRIAYSKDFETLKVDFLSKLPEmLKMFEDRLCHKTYLNGDHVTHPDFMYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPKSDLVPRGSPEFPGRLERPHR

Applications

Purification

Epitope affinity purified

Concentration

3 mg/mL

Dilution

WB:1:500-1:2,000

Form

Polyclonal antibody supplied as a 200 and 500 µl (3 mg/mL) aliquot in PBS, 20% glycerol and 0.05% sodium azide. This antibody is epitope-affinity purified from goat antiserum.

Buffer

PBS, 20% glycerol and 0.05% sodium azide

References & Citations

1. Ferreira JV, Soares AR, Ramalho J, et al. Sci Adv 2022 Mar. PMID: 35333565 2. Catarino S, Ribeiro-Rodrigues TM, Sa Ferreira R, et al. Cells 2020 Apr. PMID:32272685 3. Seixas C, Choi SY, Polgar N, et al. Mol Biol Cell. 2015 Nov 18. PMID: 26582389

Storage Conditions

For continuous use, store at 2-8 deg;C for one-two days. For extended storage, store in -20 deg;C freezer. Working dilution samples should be discarded if not used within 12 hours.