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Mouse anti M13 bacteriophage (g3p)

Product Specifications

Background

The display of repertoires of antibody fragments on the surface of filamentous phage offers a new way to produce immunoreagents with defined specificities._x000B_Phage derived antibody fragments offer a number of advantages over mouse monoclonal antibodies, such as better clearance from the blood, the possibility to select from Human combinatorial libraries and the relative ease by which such fragments can be manipulated. The phage display technique thus facilitates the selection of antibody fragments of therapeutic value or research interest._x000B_Antibodies to M13 filamentous phage coat proteins are instrumental in the selection and detection of phages expressing specific antibody fragments or peptide sequences at their surface.

UniProt

P69168 (M13 phage)

Host

Mouse

Isotype

IgG3

Clone

E1

Type

Monoclonal Antibody

Source

Hybridoma produced by the fusion of splenocytes from mice immunized with isolated M13 phage coat proteins and mouse myeloma cells.

Applications

WB, ELISA, Flow Cytometry, IHC

Field of Research

Molecular Biology

Purification Method

Protein A/G Chromatography

Assay Principle

ELISA|Western Blot|Flow Cytometry|Immunohistochemistry

Concentration

See vial for Concentration

Form

Antibody specific for the M13 bacteriophage protein coat, amongst others the g3p protein. Provided as solution in phosphate buffered saline with 0,09% sodium azide

Precautions

This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving Humans or animals.

References & Citations

1- Van Wezenbeek P.M., Schoenmakers J.G.; Nucleotide sequence of the genes III, VI and I of bacteriophage M13; Nucleic Acids Res. 6:2799-2818(1979)._x000D_ 2- Van Wezenbeek P.M., Hulsebos T.J., Schoenmakers J.G.; Nucleotide sequence of the filamentous bacteriophage M13 genome: comparison with phage fd; Gene 11:129-148(1980)._x000D_ 3- Cleary J.M., Ray D.S.; Deletion analysis of the cloned replication origin region from bacteriophage M13; J. Virol. 40:197-203(1981)._x000D_ 4- Hines J.C., Ray D.S.; Construction and characterization of new coliphage M13 cloning vectors; Gene 11:207-218(1980)._x000D_ 5- Yanisch-Perron C., Vieira J., Messing J.; Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors; Gene 33:103-119(1985)._x000D_ 6- Messing J.; New M13 Vectors for Cloning; Meth. Enzymol. 101:20 78(1983)._x000D_ 7- Sanger F., Nicklen S., Coulson A.R.; DNA sequencing with chain-terminating inhibitors; Proc. Natl. Acad. Sci. U.S.A. 74:5463-5468(1977)._x000D_ 8- Zoller M.J., Smith M.; Oligonucleotide-directed mutagenesis of DNA fragments cloned into M13 vectors; Meth. Enzymol. 100:468-500(1983)._x000D_ 9- Hu N.T., Messing J.; The making of strand-specific M13 probes; Gene 17:271-277(1982)._x000D_ 10- Heidecker G., Messing J., Gronenborn B.; A versatile primer for DNA sequencing in the M13mp2 cloning system; Gene 10:69-73(1980)._x000D_ 11- Ebright R., Dong Q., Messing J.; Corrected nucleotide sequence of M13mp18 gene III; Gene 114:81-83(1992)._x000D_ 12- Hong G.F.; A method for sequencing single-stranded cloned DNA in both directions; Biosci. Rep. 1:243-252(1981)._x000D_ 13- Messing J.; Multipurpose cloning system based on the single-stranded DNA bacteriophage M13; Recombinant DNA Technical Bulletin NIH 2:43-48(1979).

Shipping Conditions

Ambient Temperature, freeze upon arrival

Storage Conditions

Product should be stored at -20ºC; Aliquot to avoid freeze/thaw cycles

CAS Number

9007-83-4

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