Human NADPH Cytochrome P450 Reductase

<strong>Human NADPH Cytochrome P450 Reductase</strong>_x000D_ <strong>Catalog number:</strong> B2016764_x000D_ <strong>Lot number:</strong> Batch Dependent_x000D_ <strong>Expiration Date:</strong> Batch dependent_x000D_ <strong>Amount:</strong> 50 µg_x000D_ <strong>Molecular Weight or Concentration:</strong> 76.4 kDa_x000D_ <strong>Supplied as:</strong> Solution_x000D_ <strong>Applications:</strong> a molecular tool for various biochemical applications_x000D_ <strong>Storage:</strong> -80°C_x000D_ <strong>Keywords:</strong> NADPH-dependent Thioredoxin Reductase, TrxR1, Txnrd1_x000D_ <strong>Grade:</strong> Biotechnology grade. All products are highly pure. All solutions are made with Type I ultrapure water (resistivity &gt;18 MΩ-cm) and are filtered through 0.22 um._x000D_ _x000D_ <strong>References:</strong>_x000D_ 1: Park HG, Lim YR, Han S, Jeong D, Kim D. Enhanced Purification of Recombinant Rat NADPH-P450 Reductase by Using a Hexahistidine-Tag J Microbiol Biotechnol. 2017 May 28;27(5):983-989._x000D_ 2: Mishin V, Heck DE, Laskin DL, Laskin JD. Human recombinant cytochrome P450 enzymes display distinct hydrogen peroxide generating activities during substrate independent NADPH oxidase reactions Toxicol Sci. 2014 Oct;141(2):344-52._x000D_ 3: Burris-Hiday SD, Scott EE. Allosteric modulation of cytochrome P450 enzymes by the NADPH cytochrome P450 reductase FMN-containing domain J Biol Chem. 2023 Sep;299(9):105112._x000D_ 4: Khan MF, Murphy CD. Nitroreduction of flutamide by Cunninghamella elegans NADPH: Cytochrome P450 reductase Biochem Biophys Rep. 2022 Jan 17;29:101209._x000D_ 5: Szilagyi JT, Fussell KC, Wang Y, Jan YH, Mishin V, Richardson JR, Heck DE, Yang S, Aleksunes LM, Laskin DL, Laskin JD. Quinone and nitrofurantoin redox cycling by recombinant cytochrome b5 reductase Toxicol Appl Pharmacol. 2018 Nov 15;359:102-107._x000D_ 6: Shet MS, Fisher CW, Holmans PL, Estabrook RW. Human cytochrome P450 3A4: enzymatic properties of a purified recombinant fusion protein containing NADPH-P450_reductase Proc Natl Acad Sci U S A. 1993 Dec 15;90(24):11748-52._x000D_ 7: Park HG, Lim YR, Eun CY, Han S, Han JS, Cho KS, Chun YJ, Kim D. Candida albicans NADPH-P450_reductase: expression, purification, and characterization of recombinant protein Biochem Biophys Res Commun. 2010 May 28;396(2):534-8._x000D_ 8: Shaw PM, Hosea NA, Thompson DV, Lenius JM, Guengerich FP. Reconstitution premixes for assays using purified recombinant human cytochrome P450, NADPH-cytochrome P450_reductase, and cytochrome b5 Arch Biochem Biophys. 1997 Dec 1;348(1):107-15._x000D_ 9: Ramji S, Lee C, Inaba T, Patterson AV, Riddick DS. Human NADPH-cytochrome p450 reductase overexpression does not enhance the aerobic cytotoxicity of doxorubicin in human breast cancer cell lines Cancer Res. 2003 Oct 15;63(20):6914-9._x000D_ <a href="https://pubmed.ncbi.nlm.nih.gov/18838505">10: Gan L, von Moltke LL, Trepanier LA, Harmatz JS, Greenblatt DJ, Court MH. Role of NADPH-cytochrome P450 reductase and cytochrome-b5/NADH-b5 reductase in variability of CYP3A activity in human liver microsomes Drug Metab Dispos. 2009 Jan;37(1):90-6. </a>_x000D_ _x000D_ <strong>Products Related to Human NADPH Cytochrome P450 Reductase can be found at</strong> <a href="https://moleculardepot.com/product-category/Proteins/"> Proteins</a>