HIF-1 alpha Recombinant Rabbit Monoclonal Antibody [JE75-33]
Hypoxia-inducible factor 1-alpha, also known as HIF-1-alpha, is a subunit of a heterodimeric transcription factor hypoxia-inducible factor 1 (HIF-1) that is encoded by the HIF1A gene. The Nobel Prize in Physiology or Medicine 2019 was awarded for the discovery of HIF. HIF1A is a basic helix-loop-helix PAS domain containing protein, and is considered as the master transcriptional regulator of cellular and developmental response to hypoxia. The dysregulation and overexpression of HIF1A by either hypoxia or genetic alternations have been heavily implicated in cancer biology, as well as a number of other pathophysiologies, specifically in areas of vascularization and angiogenesis, energy metabolism, cell survival, and tumor invasion. Two other alternative transcripts encoding different isoforms have been identified.
Product Specifications
Product Name Alternative
Abbreviation
HIF1A, BHLHE78, MOP1, PASD8
Swiss Prot
Q16665 Human, Q61221 Mouse, O35800 Rat
Cellular Locus
Cytoplasm, Nucleus, Nucleus speckle.
Host
Rabbit
Species Reactivity
Human,Mouse,Rat
Immunogen
Recombinant protein within Human HIF-1 alpha aa 251-550 / 826.
Isotype
IgG
Clone
JE75-33
Conjugation
Non-conjugated
Type
Recombinant Rabbit monoclonal Antibody
Applications
WB, IF-Cell, IHC-P
Positive Control
Concentration
1 mg/mL
Dilution
WB: 1:5,000 ;IF-Cell: 1:100 ;IHC-P: 1:200-1:500
Purity
Protein A affinity purified.
Form
Liquid
Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Molecular Weight
Predicted band size: 93 kDa
Storage Conditions
Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.
Recombinant Antibody
Yes
CAS Number
9000-83-3
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