Anti-ALIEN Antibody

Rabbit Polyclonal Antibody specific to Alien

Product Specifications

CAS Number

9007-83-4

Product Name Alternative

SGN2, Signalosome subunit 2, Alien homolog, JAB1-containing signalosome subunit 2, Thyroid receptor-interacting protein 15, TR-interacting protein 15, TRIP-15

Gene Name

COPS2

NCBI Gene ID

UniProt

P61201

Cellular Locus

Cytoplasm, Nucleus

Host

Rabbit

Reactivity

Human, Drosophila

Immunogen

Human Alien protein.

Target Antigen

COP9 signalosome complex subunit 2

Target

ALIEN

Clonality

Polyclonal

Type

Antibody

Applications

Field of Research

Growth Factors, Cytokines, Receptors

Purification Method

Purified by immunoaffinity chromatography

Concentration

Lot Specific

Dilution

Dilute in PBS or medium that is identical to that used in the assay system.

Format

Purified

Form

Liquid

Buffer

Phosphate Buffered Saline

Function

Essential component of the COP9 signalosome complex (CSN), a complex involved in various cellular and developmental processes. The CSN complex is an essential regulator of the ubiquitin (Ubl) conjugation pathway by mediating the deneddylation of the cullin subunits of SCF-type E3 ligase complexes, leading to decrease the Ubl ligase activity of SCF-type complexes such as SCF, CSA or DDB2. The complex is also involved in phosphorylation of p53/TP53, c-jun/JUN, IkappaBalpha/NFKBIA, ITPK1 and IRF8/ICSBP, possibly via its association with CK2 and PKD kinases. CSN-dependent phosphorylation of TP53 and JUN promotes and protects degradation by the Ubl system, respectively. Involved in early stage of neuronal differentiation via its interaction with NIF3L1. {PubMed:11285227, PubMed:11337588, PubMed:12628923, PubMed:12732143, PubMed:9535219}.

Additionnal Information

Immunoblotting: use at 1:500-1:1,000 dilution. In immunoblots, a band of approximately 50 kD is detected.

Storage Conditions

This antibody is stable for at least one (1) year at -20°C. Avoid multiple freeze- thaw cycles.

Specificity

This antibody recognizes human and Drosophila Alien, a co-repressor protein that interacts with the thyroid hormone receptor in the absence of hormone. Alien is highly conserved between human and Drosophila and is specific for selected members of the nuclear hormone receptor superfamily.