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Anti-MDC1 Antibody

Mouse Monoclonal Antibody specific to MDC1

Product Specifications

Product Name Alternative

Anti-MDC1 Mouse Monoclonal Antibody

Gene Name

Mdc1

NCBI Gene ID

<a href="https://www.ncbi.nlm.nih.gov/gene/?term=Mdc1">Mdc1</a>

UniProt

Q5PSV9

Accession Number

NP_001010833.2

Cellular Locus

Nucleus. Chromosome. Note=Associated with chromatin. Relocalizes to discrete nuclear foci following DNA damage, this requires phosphorylation of H2AX. Colocalizes with APTX at sites of DNA double-strand breaks (By similarity).

Host

Mouse

Reactivity

Human, Mouse, Bovine, Chimpanzee

Immunogen

GST-tagged recombinant mouse MDC1 (accession no. NP_001010833.2).

Target Antigen

Mediator of DNA damage checkpoint protein 1

Target

MDC1

Clonality

Monoclonal

Isotype

IgG1

Type

Antibody

Applications

WB, ICC/IF

Field of Research

DNA

Purification Method

Purified by Protein G affinity chromatography

Concentration

1.0 mg/mL

Dilution

Dilute in PBS or medium which is identical to that used in the assay system.

Format

Purified

Form

Liquid

Buffer

Phosphate Buffered Saline

Function

Required for checkpoint mediated cell cycle arrest in response to DNA damage within both the S phase and G2/M phases of the cell cycle. May serve as a scaffold for the recruitment of DNA repair and signal transduction proteins to discrete foci of DNA damage marked by 'Ser-139' phosphorylation of histone H2AX. Also required for downstream events subsequent to the recruitment of these proteins. These include phosphorylation and activation of the ATM, CHEK1 and CHEK2 kinases, and stabilization of TP53 and apoptosis. ATM and CHEK2 may also be activated independently by a parallel pathway mediated by TP53BP1 (By similarity). {ECO:0000250}.

Additionnal Information

Immunocytochemistry Immunoblotting: use at 1ug/mL. A band of ~184kDa is detected. Note: There are 4 isoforms of human MDC1 with molecular weights of approximately 227, 198, 196, and 117kDa.(For more information see Lou et al. 2003, Nature 421: 957-961) <br><br>These are recommended concentrations; <br><br>Enduser should determine optimal concentrations for their applications. <br><br>Positive control: HeLa cell lysate.

Storage Conditions

This antibody is stable for at least one (1) year at -20°C.

Product Datasheet

https://www.qedbio.com/wp-content/uploads/pdf/12596.pdf

Specificity

This antibody recognizes human, mouse, bovine and chimpanzee MDC1. Specific epitope is at the N-terminus of MDC1.

Formulation

PBS, pH 7.4, 50% glycerol, 0.09% sodium azide.Purified by Protein G affinity chromatography.

Buffer pH

pH 7.4

Target Background

Recent studies have shown that MDC1 (mediator of DNA damage checkpoint protein1) regulates many aspects of DNA damage response pathways, such as intra-S phase checkpoint, G2/M checkpoint, and radiation-induced apoptosis. Many proteins, such as ATM, BRCA1, and Chk2, interact with MDC1. MDC1 contains several protein-protein interaction domains. MDC1 appears to function as an adaptor protein, recruiting downstream proteins to upstream kinases and facilitating signal transduction following DNA damage.

CAS Number

9007-83-4

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