Rat Bladder Epithelial Cells

Rat Primary Bladder Epithelial Cells from AcceGen are isolated from bladder tissue of 6-8 week old laboratory Sprague–Dawley rat. Rat Primary Bladder Epithelial Cells are grown in T25 tissue culture flask pre-coated with gelatin-based coating solution for 2 min and incubated in AcceGen's Cell Culture Medium for 3-5 days. Cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains 0.5x106 cells and is delivered frozen. The method we use to isolate primary epithelial cells was developed based on a combination of established and our proprietary methods. These cells are pre-coated with EpCAM (CD326) antibody, following the application of magnetic beads pre-coated with secondary antibody.