C57BL/6 Mouse Primary Tracheal and Bronchial Epithelial Cells

C57BL/6 Mouse Primary Tracheal and Bronchial Epithelial Cells from AcceGen are isolated from tracheal and bronchial tissue of pathogen-free laboratory mice. C57BL/6 Mouse Primary Tracheal and Bronchial Epithelial Cells are grown in a T25 tissue culture flask pre-coated with gelatin-based coating solution for 2 min and incubated in AcceGen’ Culture Complete Growth Medium for 3-5 days. Cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 0.5×106 cells per ml and is delivered frozen. The method we use to isolate primary epithelial cells was developed based on a combination of established and our proprietary methods. These cells are pre-coated with EpCAM-1 (CD326) antibody, following the application of magnetic beads pre-coated with secondary antibody.