Human Villous Trophoblasts

The trophoblast begins at the outer covering of the early blastocyst and provides the route of nourishment between the maternal endometrium and the developing embryo. The trophoblast adhesion to the uterine wall is the requisite first step of implantation and, subsequently, placentation. Human villous tryophoblasts (HVT) covering the villi of the placenta provide the surface for the exchange of oxygen and nutrient with the maternal circulation. They express CXCR4, CCR5 and prolactin gene family. They acquire CCR1 as they differentiate to an invasive phenotype at the villous-anchoring sites. The feature of HVT together with the recent establishment of trophoblast stem cells makes them an ideal genetic platform to study cell differentiation and organogenesis.HVT from AcceGen Research Laboratories are isolated from human placental villi. HVT are cryopreserved at passage primary culture and delivered frozen. Each vial contains >1 x 10^6 cells in 1 ml volume. HVT are characterized by immunofluorescent method with antibodies to alpha-and beta-HCG. HVT are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HVT are guaranteed to further culture the condition provided by AcceGen Research Laboratories.