Human Splenic Fibroblasts

Fibroblasts are mesenchymal cells derived from the embryonic mesoderm. They have been extensively used for a wide range of cellular and molecular studies. This is mainly because they are one of easiest types of cells to grow in culture, and their durability makes them amenable to a wide variety of manipulations ranging from studies employing gene transfection to microinjection. Fibroblasts within tissues are exposed to a dynamic mechnical environment, which influences the structure integrity of both healthy and healing soft tissue. They secrete a non-rigid extracellular matrix that is rich in type I and/or type III collagen. There is good evidence that fibroblasts in different parts of the body are intrinsically different and display different regulatory functions on surrounding cells. For example, splenic fibroblasts have been shown to regulate the development of NK cells from blood progenitors through an expression of bioactive IL-15. HSF from AcceGen Research Laboratories are isolated from human spleen tissue. HSF are cryopreserved at primary culture and delivered frozen. Each vial contains >5×10^5 cells in 1 ml volume. HSF are characterized by their spindle morphology and immunofluorescent method with antibody to fibronectin. HSF are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HSF are guaranteed to further expand for 15 population doublings at the condition provided by AcceGen Research Laboratories.