Human Skeletal Muscle Myoblasts

Skeletal muscle regenerates by the proliferation of mononuclear myogenic precursor cells called myoblasts that ultimately fuse and become incorporated into multinucleated myotubes, which later mature into myofibres. This process occurs during the embryonic histogenesis of muscle and in postnatal muscle regenerating in response to injury, or in myopathies such as Duchenne muscular dystrophy. The fusion of myoblasts is specific to skeletal muscel. Myoblasts that do not form muscle fibers differentiate into satellite cells. Skeletal muscel myoblasts express FGF receptor and IGF expression is increased during myoblast differentiation in culture. The human skeletal muscle myoblast culture is a convenient in vitro model for the study of cellular development and differentiation, RI matablism and tissue repair.HSkMM from AcceGen Research Laboratories are isolated from human muscle of the pectoral girdle. HSkMM are cryopreserved on primary culture or passage one and delivered frozen. Each vial contains >5×10^5 cells in 1 ml volume. HSkMM are characterized by immunofluorescent method with antibodies to myosin, actin and actinin. HSkMM are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HSkMM are guaranteed to further expand for 15 population doublings at the conditions provided by AcceGen Research Laboratories.