Human Renal Mesangial Cells

Renal mesangial cells (RMC) are perivascular pericytes located within the central portion of the glomerular tuft between capillary loops. Mesangial cells have a variety of functions including synthesis and assembly of the mesangial matrix, endocytosis and processing of plasma macromolecules, and control of glomerular hemodynamics via mesangial cell contraction or release of vasoactive hormones. Mesangial cell proliferation and matrix overproduction are the predominant pathological features of many forms of glomerulonephritis, such as IgA nephropathy, lupus nephropathy, and diabetic nephropathy and frequently precedes the increase of extracellular matrix in the mesangium and the development of glomerulosclerosis. As proliferation and matrix synthesis/degradation in vitro are regulated by cytokines and growth factors, cultured cells are an ideal tool for studying pathophysiological events such as mesangial expansion, scarring, and glomerulosclerosis.HRMC from AcceGen Research Laboratories are isolated from human renal tissue. HRMC are cryopreserved after purification and delivered frozen. Each vial contains >5×10^5 cells in 1 ml volume. HRMC are characterized by immunofluorescent method with antibodies to fibronectin, Thy-1, and smooth muscle actin. HRMC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HRMC are guaranteed to further expand for 15 population doublings at the conditions specified by AcceGen Research Laboratories.