Human Conjunctival Fibroblasts

The fibroblast is the cellular element responsible for fibrosis. Conjunctival fibroblasts, as other fibroblasts, act not only as structural cells in the production of the matrix components, but are also actively involved as effectors in allergic conjunctivitis. Conjunctival fibroblasts cultured with histamine induce the production of proinflammatory cytokines, such as IL-1, IL-6 and IL-8. Conjunctival fibroblast culture can serve as a useful in vitro model for rapid screening of drugs for their effect on fibroblast morphology, motility, and proliferation, and provide a basis for the development of new treatments for a variety of ocular proliferative disorders.HConF from AcceGen Research Laboratories are isolated from human conjunctiva. HConF are cryopreserved at primary culture and delivered frozen. Each vial contains >5×10^5 cells in 1 ml volume. HConF are characterized by their spindle morphology and immunofluorescent method with antibody to fibronectin. HConF are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HConF are guaranteed to further expand for 15 population doublings at the condition provided by AcceGen Research Laboratories.