Human Cardiac Fibroblasts

The cardiac fibroblasts (CF) are the principal cell type of the heart. They provide structural support for cardiac myocytes and are responsible for extracellular matrix synthesis in the heart during growth and pathophysiological conditions. CF are an important cellular component of myocardial responses to injury and the source of paracrine growth factors. Their proliferation and synthesis of matrix is essential for scar formation at sites of myocardial infarction, cardiac fibrosis, and is often complicated by cardiac hypertrophy. CF also respond to changing environments in pathophysiological conditions by modulating integrin expression. CF culture has been widely used as a model to study the cardiac matrix remodeled by physiological (exercise) and pathological (hypertension) stressors.HCF from AcceGen Research Laboratories are isolated from the human heart. HCF-a are cryopreserved at secondary culture and delivered frozen. Each vial contains >5×10^5 cells in 1 ml volume. HCF-a are characterized by immunofluorescent method with antibody to fibronectin. HCF-a are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HCF-a are guaranteed to further expand for 15 population doublings at the condition provided by AcceGen Research Laboratories.