Anti-dsRNA monoclonal antibody J2
Anti-dsRNA monoclonal antibody J2 is the gold standard for the detection of dsRNA both in cultured cells and fixed paraffin-embedded histological samples.
Product Specifications
CAS Number
9007-83-4
Specifications
Anti-dsRNA monoclonal antibody J2 recognises double-stranded RNA (dsRNA) provided that the length of the helix is greater than or equal to 40 bp. dsRNA-recognition is independent of the sequence and nucleotide composition of the antigen. All naturally occurring dsRNAs investigated up to now (40-50 species) as well as poly(I)-poly(C) and poly(A)-poly(U) have been recognised by Anti-dsRNA monoclonal antibody J2 although in some assays its affinity to poly(I)-poly(C) is about 10 times lower than that to other dsRNA antigens.
Product Name Alternative
Mouse, IgG2a, kappa chain
Synonyms
mouse, IgG2a, kappa chain
UNSPSC
12352203
UNSPSC Description
Antibodies
HS Code
38221900
eClass Number
32160702
eClass Product Group
Antigen, Antikörper (Labor)
Applications
ELISA, IF, FACS, IHC, IP, Dot Blot, ChIP, affinity purification, immunoelectron microscopy.
Form
lyophilised from a 1 mg/mLSolution in PBS
Autoclavability
false
Solubility
To prepare a 1 μg/μl PBS antibody solution add 200 μl (RNT-SCI-10010200) or 500 μl (RNT-SCI-10010500) sterile DNAse/RNAse-free distilled Water. As a result of the lyophilisation procedure, the reconstituted antibody may contain small amounts of denatured protein in the form of aggregates that may interfere with some applications such as immunohistochemistry (e.g. by giving high backgrounds). We therefore highly recommend to spin down (microcentrifuge) the reconstituted antibody before use and to use the supernatant only.
Shipping Conditions
Shipped on gel packs
Storage Conditions
Avoid freeze/thaw cycles, store in aliquot.
Storage Temperature
Store at -20 °C to -80 °C upon reconstitution for long-term storage
Notes
After adding 10 mM sodium azide the undiluted antibody (1 µg/µl) can be stored at 4°C for a short period of time
Shelf Life
12 months after date of delivery
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