Anti-dsRNA monoclonal antibody J2

Anti-dsRNA monoclonal antibody J2 is the gold standard for the detection of dsRNA both in cultured cells and fixed paraffin-embedded histological samples.

Product Specifications

CAS Number

9007-83-4

Specifications

Anti-dsRNA monoclonal antibody J2 recognises double-stranded RNA (dsRNA) provided that the length of the helix is greater than or equal to 40 bp. dsRNA-recognition is independent of the sequence and nucleotide composition of the antigen. All naturally occurring dsRNAs investigated up to now (40-50 species) as well as poly(I)-poly(C) and poly(A)-poly(U) have been recognised by Anti-dsRNA monoclonal antibody J2 although in some assays its affinity to poly(I)-poly(C) is about 10 times lower than that to other dsRNA antigens.

Product Name Alternative

Mouse, IgG2a, kappa chain

Synonyms

mouse, IgG2a, kappa chain

UNSPSC

12352203

UNSPSC Description

Antibodies

HS Code

38221900

eClass Number

32160702

eClass Product Group

Antigen, Antikörper (Labor)

Applications

ELISA, IF, FACS, IHC, IP, Dot Blot, ChIP, affinity purification, immunoelectron microscopy.

Form

lyophilised from a 1 mg/mLSolution in PBS

Autoclavability

false

Solubility

To prepare a 1 μg/μl PBS antibody solution add 200 μl (RNT-SCI-10010200) or 500 μl (RNT-SCI-10010500) sterile DNAse/RNAse-free distilled Water. As a result of the lyophilisation procedure, the reconstituted antibody may contain small amounts of denatured protein in the form of aggregates that may interfere with some applications such as immunohistochemistry (e.g. by giving high backgrounds). We therefore highly recommend to spin down (microcentrifuge) the reconstituted antibody before use and to use the supernatant only.

Shipping Conditions

Shipped on gel packs

Storage Conditions

Avoid freeze/thaw cycles, store in aliquot.

Storage Temperature

Store at -20 °C to -80 °C upon reconstitution for long-term storage

Notes

After adding 10 mM sodium azide the undiluted antibody (1 µg/µl) can be stored at 4°C for a short period of time

Shelf Life

12 months after date of delivery

Documents

Product Datasheet

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Product MSDS

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