HighYield T7 sgRNA Synthesis Kit (SpCas9)

HighYield T7 sgRNA Synthesis Kit (SpCas9) is designed for cloning-free synthesis of SpCas9-specific single-guided RNAs (sgRNAs) via in vitro transcription. SgRNAs direct sequence-specific DNA recognition once complexed with Streptococcus pyogenes Cas9 (SpCas9)[1,2]. The resulting sgRNA/SpCas9 ribonucleoprotein (RNP) complex can thus be used for site-specific cleavage, nicking or binding of dsDNA both in vitro and in living cells depending on the choice of SpCas9 variant (e.g. wildtype, nickase (D10A), nuclease deficient(D10A/H840A)). Cleavage, nicking or binding of SpCas9 variant occurs upstream of the SpCas9-specific DNA recognition sequence 5'-NGG-3' (protospacer adjacent motif (PAM) sequence, N = any nucleotide base).