Extreme Thermolabile UNG (Uracil N-Glycosylase)

Extreme Thermolabile UNG (UDG) is used in one-step RT-PCR systems to prevent carry-over contamination with dU-containing DNA from previous reactions prior to the reverse transcription step. The enzyme is fully active at temperatures between 15 to 25°C and inactivated at 50°C. Extreme Thermolabile UNG is a recombinant uracil-DNA glycosylase from marine bacterium BMTU 3346. UNG catalyzes the hydrolysis of the N-glycosidic bond between the uracil and sugar, leaving an apyrimidinic site in uracil-containing single or double-stranded DNA. An amount of 1 unit UNG completely digests 104 to 106 copies of U-containing DNA fragments in 2 min at 50°C.