Thermolabile UNG (Uracil N-Glycosylase) - 200 u/µl

Thermolabile UNG - high conc. is recommended for use in freeze drying applications or in applications where only a minimal amount of volume can be used. The remaining glycerol concentration in the final assay is neglectable due to a high dilution factor. UNG is used in real-time PCR to prevent carry-over contamination of dU-containing DNA from previous reactions. Uracyl N-Glycosylase (UNG, UDG) catalyzes the release of uracil from single and double stranded uracyl-containing DNA. The resulting abasic sites are susceptible to hydrolytic cleavage at elevated temperatures. An amount of 1 unit UNG completely digests 104 to 106 copies of U-containing DNA fragments in 2 min at 50°C.