Thermolabile UNG (Uracil N-Glycosylase) - 1 u/µl
Thermolabile UNG is used in real-time PCR to prevent carry-over contamination of dU-containing DNA from previous reactions. Uracyl N-Glycosylase (UNG, UDG) catalyses the release of uracil from single and double stranded uracyl-containing DNA. The resulting abasic sites are susceptible to hydrolytic cleavage at elevated temperatures. An amount of 1 unit UNG completely digests 104 to 106 copies of U-containing DNA fragments in 2 min at 50°C.
Product Specifications
Synonyms
Prevention of carry-over contaminations
UNSPSC
41106300
UNSPSC Description
Polymerase chain reaction PCR and reverse transcriptase polymerase chain reaction RT PCR products
HS Code
35079090
eClass Number
32160502
eClass Product Group
PCR-Reagenz labor
Concentration
1 unit/μL
Form
Liquid (Supplied in 20 mM Tris-HCl pH 8.0, 50 mM NaCl,1 mM EDTA, 1 mM DTT, 100 μg/ml BSA and 50 % [v/v] glycerol)
Autoclavability
false
Shipping Conditions
Shipped on gel packs
Storage Conditions
Store at -20 °C Avoid freeze/thaw cycles
Shelf Life
12 months
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