QPCR GreenMaster Lyophilisate

qPCR GreenMaster Lyophilisate is designed for quantitative real-time analysis of DNA samples using a green-fluorescent DNA stain structurally similar to SYBR® GREEN. The fluorescent dye in the master mix intercalates into the amplification product during the PCR process and enables the rapid analysis of target DNA without the need to synthesize sequence-specific labeled probes. It provides an easy-to-handle and powerful tool for quantification of sample DNA in a broad dynamic range of up to 6 orders of magnitude with exceptional sensitivity and precision. The lyophilisate contains all reagents required for qPCR (except template, primer and labeled fluorescent probe) in a single bead. The high specificity and sensitivity of the mix based on an optimized hot-start polymerase. Its activity is blocked at ambient temperature and switched on automatically at the onset of the initial denaturation. The thermal activation prevents the extension of nonspecifically annealed primers and primer-dimer formation at low temperatures during PCR setup.