Fluorescein-12-dCTP

Fluorescein-12-dCTP is recommended for direct enzymatic labeling of DNA/cDNA e.g. by PCR and Nick Translation. It is incorporated as substitute for its natural counterpart dCTP. The resulting Dye-labeled DNA/cDNA probes are ideally suited for fluorescence hybridization applications such as FISH or microarray-based gene expression profiling. Optimal substrate properties and thus labeling efficiency is ensured by an optimized linker attached to the C5 position of cytidine.

Product Specifications

Product Name Alternative

Fluorescein-X-5-Propargylamino-dCTP

Synonyms

Fluorescein-X-5-Propargylamino-dCTP

UNSPSC

12352208

UNSPSC Description

Nucleic acids

HS Code

29349990

eClass Number

34160990

eClass Product Group

Nucleinsäure (nicht klassifiziert)

Applications

Incorporation into DNA/cDNA by - PCR with Taq polymerase in-house data - Nick Translation with DNAse I/ DNA Polymerase I in-house data

Concentration

(1 mM)

Purity

≥ 95 % (HPLC)

Form

Solution in 10 mM Tris-HCl

pH

7.5 ±0.5

Color

Yellow-orange

Autoclavability

false

Smiles

[C@@H]1(C[C@@H](O[C@@H]1COP(=O)(OP(=O)(OP(=O)(O)O)O)O)n1c(=O)nc(c(c1)C#CCNC(=O)CCCCCNC(=O)c1ccc(c(c1)c1c2c(oc3c1ccc(=O)c3)cc(cc2)O)C(=O)O)N)O.[C@@H]1(C[C@@H](O[C@@H]1COP(=O)(OP(=O)(OP(=O)(O)O)O)O)n1c(=O)nc(c(c1)C#CCNC(=O)CCCCCNC(=O)c1ccc(c(c1)C(=O)O)c1c2c(oc3c1ccc(=O)c3)cc(cc2)O)N)O