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Fluorescein-12-dCTP

Fluorescein-12-dCTP is recommended for direct enzymatic labeling of DNA/cDNA e.g. by PCR and Nick Translation. It is incorporated as substitute for its natural counterpart dCTP. The resulting Dye-labeled DNA/cDNA probes are ideally suited for fluorescence hybridization applications such as FISH or microarray-based gene expression profiling. Optimal substrate properties and thus labeling efficiency is ensured by an optimized linker attached to the C5 position of cytidine.

Product Specifications

Product Name Alternative

Fluorescein-X-5-Propargylamino-dCTP

UNSPSC

12352208

UNSPSC Description

Nucleic acids

HS Code

29349990

eClass Number

34160990

eClass Product Group

Nucleinsäure (nicht klassifiziert)

Applications

Incorporation into DNA/cDNA by - PCR with Taq polymerase in-house data - Nick Translation with DNAse I/ DNA Polymerase I in-house data

Concentration

(1 mM)

Purity

≥ 95 % (HPLC)

Form

Solution in 10 mM Tris-HCl

pH

7.5 ±0.5

Color

Yellow-orange

Autoclavability

false

Smiles

[C@@H]1 (C[C@@H] (O[C@@H]1COP (=O) (OP (=O) (OP (=O) (O) O) O) O) n1c (=O) nc (c (c1) C#CCNC (=O) CCCCCNC (=O) c1ccc (c (c1) c1c2c (oc3c1ccc (=O) c3) cc (cc2) O) C (=O) O) N) O.[C@@H]1 (C[C@@H] (O[C@@H]1COP (=O) (OP (=O) (OP (=O) (O) O) O) O) n1c (=O) nc (c (c1) C#CCNC (=O) CCCCCNC (=O) c1ccc (c (c1) C (=O) O) c1c2c (oc3c1ccc (=O) c3) cc (cc2) O) N) O

Shipping Conditions

Shipped on gel packs

Storage Conditions

Store at -20 °C. Short term exposure (up to 1 week cumulative) to ambient temperature possible.

Storage Temperature

Store at -20 °C

Notes

Short term exposure (up to 1 week cumulative) to ambient temperature possible.

Shelf Life

12 months after date of delivery

Product Datasheet

https://cdn.gentaur.com/products/220/68dd0951061270361e3ddb23/datasheet/nu-809-famx-l.pdf

Product MSDS

https://cdn.gentaur.com/products/220/68dd0951061270361e3ddb23/msds/nu-809-famx-l.pdf

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