Biotin-11-dCTP
Biotin-11-dCTP is enzymatically incorporated into DNA/cDNA as substitute for its natural counterpart dCTP. The resulting Biotin-labeled DNA/cDNA probes are subsequently detected using streptavidin conjugated with horseradish peroxidase (HRP), alkaline phosphatase (AP), a fluorescent dye or agarose/magnetic beads. Optimal substrate properties and thus labeling efficiency as well as an efficient detection of the Biotin moiety is ensured by a 11-atom linker attached to the C5 position of cytidine.
Product Specifications
CAS Number
136632-30-9
Product Name Alternative
Biotin-X-5-Propargylamino-dCTP
Synonyms
Biotin-X-5-Propargylamino-dCTP
UNSPSC
12352208
UNSPSC Description
Nucleic acids
HS Code
29349990
eClass Number
34160990
eClass Product Group
Nucleinsäure (nicht klassifiziert)
Applications
FISH[1, 2] SNP-analysis[1] Nick-translation[2] TUNEL[3]
Concentration
(1 mM)
Purity
≥ 95 % (HPLC)
Form
Filtered solution (30 kDa) in 10 mM Tris-HCl
pH
7.5 ±0.5
Color
Colorless to slightly yellow
Autoclavability
false
Smiles
N(CC#Cc1c(nc(=O)n([C@H]2C[C@@H]([C@H](O2)COP(=O)(OP(=O)(OP(=O)(O)O)O)O)O)c1)N)C(=O)CCCCCNC(=O)CCCC[C@H]1[C@H]2NC(=O)N[C@H]2CS1
Shipping Conditions
Shipped on gel packs
Storage Conditions
Store at -20 °C Short term exposure up to 1 week cumulative to ambient temperature possible.
Storage Temperature
Store at -20 °C
Notes
Short term exposure (up to 1 week cumulative) to ambient temperature possible.
Shelf Life
12 months after date of delivery
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