Biotin-16-dCTP

Biotin-16-dCTP is enzymatically incorporated into DNA/cDNA as substitute for its natural counterpart dCTP. The resulting Biotin-labeled DNA/cDNA probes are subsequently detected using streptavidin conjugated with horseradish peroxidase (HRP), alkaline phosphatase (AP), a fluorescent dye or agarose/magnetic beads. Optimal substrate properties and thus labeling efficiency as well as an efficient detection of the Biotin moiety is ensured by a 16-atom linker attached to the C5 position of cytidine.

Product Specifications

Product Name Alternative

Biotin-16-Propargylamino-dCTP

Synonyms

Biotin-16-Propargylamino-dCTP

UNSPSC

12352208

UNSPSC Description

Nucleic acids

HS Code

29349990

eClass Number

34160990

eClass Product Group

Nucleinsäure (nicht klassifiziert)

Applications

Incorporation into DNA/cDNA by - PCR with Taq polymerase in-house data - Nick Translation with DNAse I/ DNA Polymerase I [1,2] - Primer Extension with Klenow exo- [3] - 3’-End Labeling with Terminal deoxynucleotidyl Transferase (TdT) [4] - Reverse Transcription with MMLV Reverse Transcriptase [5]

Concentration

(1 mM)

Purity

≥ 95 % (HPLC)

Form

Filtered solution (30 kDa) in 10 mM Tris-HCl

pH

7.5 ±0.5

Color

Colorless to slightly yellow

Autoclavability

false

Smiles

C1[C@H]2[C@@H] ([C@@H] (S1) CCCCC (=O) NCCCCCC (=O) NCCCC (=O) NCC#Cc1cn (c (=O) nc1N) [C@@H]1O[C@H] (COP (=O) (O) OP (=O) (O) OP (=O) (O) O) [C@@H] (O) C1) NC (=O) N2

Shipping Conditions

Shipped on gel packs

Storage Conditions

Store at -20 °C Short term exposure up to 1 week cumulative to ambient temperature possible.

Storage Temperature

Store at -20 °C

Notes

Short term exposure (up to 1 week cumulative) to ambient temperature possible.

Shelf Life

12 months after date of delivery

Product Datasheet

https://cdn.gentaur.com/products/220/68dd094e061270361e3dcb7a/datasheet/nu-809-bio16-s.pdf

Product MSDS

https://cdn.gentaur.com/products/220/68dd094e061270361e3dcb7a/msds/nu-809-bio16-s.pdf

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