Aminoallyl-dUTP-XX-Cy3

Aminoallyl-dUTP-XX-Cy3 is recommended for direct enzymatic labeling of DNA/cDNA e.g. by PCR and Nick Translation. It is incorporated as substitute for its natural counterpart dTTP. The resulting Dye-labeled DNA/cDNA probes are ideally suited for fluorescence hybridization applications such as FISH or microarray-based gene expression profiling. Optimal substrate properties and thus labeling efficiency is ensured by an optimized linker attached to the C5 position of uridine. Higher incorporation and thus labeling rates can be achieved compared to Aminoally-dUTP-Cy3 (NU-803-Cy3).

Product Specifications

UNSPSC

12352208

UNSPSC Description

Nucleic acids

HS Code

29349990

eClass Number

34160990

eClass Product Group

Nucleinsäure (nicht klassifiziert)

Applications

Incorporation into DNA/cDNA by - PCR with Taq polymerase in-house data - Nick Translation with DNAse I/ DNA Polymerase I in-house data

Concentration

(1 mM)

Purity

≥ 95 % (HPLC)

Form

Filtered solution (30 kDa) in 10 mM Tris-HCl

pH

7.5 ±0.5

Color

Pink

Autoclavability

false

Smiles

C(/C=C/c1cn(c(=O)[nH]c1=O)[C@H]1C[C@@H]([C@H](O1)COP(=O)(O)OP(=O)(O)OP(=O)(O)O)O)NC(=O)CCCCCNC(=O)CCCCCNC(=O)CCCCCN1c2ccc(S(=O)(=O)O)cc2C(C)(C)/C/1=C\C=C\C1=[N+](c2ccc(cc2C1(C)C)S(=O)(=O)[O-])CC