Aminoallyl-dUTP-5/6-TAMRA

Aminoallyl-dUTP-5/6-TAMRA is recommended for direct enzymatic labeling of DNA/cDNA by Nick Translation. It is incorporated as substitute for its natural counterpart dTTP. The resulting Dye-labeled DNA/cDNA probes are ideally suited for fluorescence hybridization applications such as FISH or microarray-based gene expression profiling. Optimal substrate properties and thus labeling efficiency is ensured by an optimized linker attached to the C5 position of uridine.

Product Specifications

Product Name Alternative

Tetramethyl-Rhodamine-5-dUTP

Synonyms

Tetramethyl-Rhodamine-5-dUTP

UNSPSC

12352208

UNSPSC Description

Nucleic acids

HS Code

29349990

eClass Number

34160990

eClass Product Group

Nucleinsäure (nicht klassifiziert)

Applications

Incorporation into DNA/cDNA by - Nick Translation with DNAse I/ DNA Polymerase I in-house data, [1,2]

Concentration

(1 mM)

Purity

≥ 95 % (HPLC)

Form

Filtered solution (30 kDa) in 10 mM Tris-HCl

pH

7.5 ±0.5

Color

Pink to red

Autoclavability

false

Smiles

C(/C=C/c1cn(c(=O)[nH]c1=O)[C@H]1C[C@@H]([C@H](O1)COP(=O)(O)OP(=O)(O)OP(=O)(O)O)O)NC(=O)c1cc(c(cc1)C(=O)[O-])c1c2c(oc3c1ccc(c3)N(C)C)cc(=[N+](C)C)cc2.C(/C=C/c1cn(c(=O)[nH]c1=O)[C@H]1C[C@@H]([C@H](O1)COP(=O)(O)OP(=O)(O)OP(=O)(O)O)O)NC(=O)c1ccc(c(c1)C(=O)[O-])c1c2c(oc3c1ccc(c3)N(C)C)cc(=[N+](C)C)cc2