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Aminoallyl-dUTP - Solution

Aminoallyl-dUTP is recommended for two-step labeling of DNA/cDNA e.g. by PCR, Nick Translation, Primer Extension, 3'-End Labeling and Reverse Transcription. It is enzymatically incorporated into DNA/cDNA as substitute for its natural counterpart dTTP. The resulting Amine-functionalized DNA/cDNA can subsequently be labeled via the classic Amine/NHS Ester reaction that offers the choice to introduce a Biotin group (via NHS Ester of Biotin) for subsequent purification tasks to introduce fluorescent group (via NHS Ester of fluorescent dyes) for subsequent microscopic imaging

Product Specifications

UNSPSC

12352208

UNSPSC Description

Nucleic acids

HS Code

29349990

eClass Number

34160990

eClass Product Group

Nucleinsäure (nicht klassifiziert)

Applications

Incorporation into DNA/cDNA by PCR with Taq polymerase[1], in-house data Nick Translation with DNAse I/ DNA Polymerase I[2] Primer Extension with Klenow exo- [3] 3'-End Labeling with Terminal deoxynucleotidyl Transferase (TdT)[4] Reverse Transcription with MMLV Reverse Transcriptase[2]

Concentration

(100 mM)

Purity

≥ 95 % (HPLC)

Form

Solution in water

pH

7.5 ±0.5

Color

Colorless to slightly yellow

Autoclavability

false

Smiles

NC/C=C/c1cn (c (=O) [nH]c1=O) [C@H]1C[C@@H] ([C@H] (O1) COP (=O) (O) OP (=O) (O) OP (=O) (O) O) O

Shipping Conditions

Shipped on gel packs

Storage Conditions

Store at -20 °C. Short term exposure (up to 1 week cumulative) to ambient temperature possible.

Storage Temperature

Store at -20 °C

Notes

Short term exposure (up to 1 week cumulative) to ambient temperature possible.

Shelf Life

12 months after date of delivery

Product Datasheet

https://cdn.gentaur.com/products/220/68dd0950061270361e3dd822/datasheet/nu-803l.pdf

Product MSDS

https://cdn.gentaur.com/products/220/68dd0950061270361e3dd822/msds/nu-803l.pdf

CAS Number

116840-18-7

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