Fluorescein-12-dUTP

Fluorescein-12-dUTP is recommended for direct enzymatic labeling of DNA/cDNA e.g. by PCR and Nick Translation. It is incorporated as substitute for its natural counterpart dTTP. The resulting Dye-labeled DNA/cDNA probes are ideally suited for fluorescence hybridization applications such as FISH or microarray-based gene expression profiling.Optimal substrate properties and thus labeling efficiency is ensured by an optimized linker attached to the C5 position of uridine. Recommended Fluorescein-12-dUTP/dTTP ratio for PCR and Nick Translation: 30-50% Fluorescein-12-dUTP/ 70-50% dTTP

Product Specifications

Product Name Alternative

Fluorescein-X- (5-aminoallyl) -dUTP

UNSPSC

12352208

UNSPSC Description

Nucleic acids

HS Code

29349990

eClass Number

34160990

eClass Product Group

Nucleinsäure (nicht klassifiziert)

Applications

Incorporation into DNA/cDNA by - PCR with Taq polymerase in-house data, [1] - Nick Translation with DNAse I/ DNA Polymerase I in-house data

Concentration

(1 mM)

Purity

≥ 95 % (HPLC)

Form

Solution in water

pH

7.5 ±0.5

Color

Yellow-orange

Autoclavability

false

Smiles

O=c1ccc2c(c1)oc1cc(O)ccc1c2c1c(ccc(c1)C(=O)NCCCCCC(=O)NC/C=C/c1cn(c(=O)[nH]c1=O)[C@@H]1O[C@H](COP(=O)(O)OP(=O)(O)OP(=O)(O)O)[C@@H](O)C1)C(=O)O.O=c1c(cn(c(=O)[nH]1)[C@@H]1O[C@H](COP(=O)(O)OP(=O)(O)OP(=O)(O)O)[C@@H](O)C1)/C=C/CNC(=O)CCCCCNC(=O)c1cc(c(c2c3ccc(O)cc3oc3cc(=O)ccc23)cc1)C(=O)O